OLFM4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH01-37] (HA750715)
Catalog# HA750715
Rabbit Monoclonal to OLFM4
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of OLFM4 on different lysates with Rabbit anti-OLFM4 antibody (HA750715) at 1/1,000 dilution.
Lane 1: OLFM4 recombinant protein
Lane 2: OLFM4 recombinant protein with immunising peptide
Lysates/proteins at 50 ng/Lane.
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750715) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Lane 1: OLFM4 recombinant protein
Lane 2: OLFM4 recombinant protein with immunising peptide
Lysates/proteins at 50 ng/Lane.
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750715) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.