GANAB Recombinant Rabbit Monoclonal Antibody [PSH01-52] - BSA and Azide free
Catalog# HA750728
GANAB Recombinant Rabbit Monoclonal Antibody [PSH01-52] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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IP
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Human
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Mouse
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Rat
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HA721695
含抗保成分
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unconjugated
Overview
Product Name
GANAB Recombinant Rabbit Monoclonal Antibody [PSH01-52] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human GANAB aa 29-450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue, IP
Molecular Weight
Predicted band size: 107 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, Raji cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, human thyroid tissue, LoVo cell lysate, U-2 OS cell lysate, K-562 cell lysate, Jurkat cell lysate, HL-60 cell lysate, RAW264.7 cell lysate, mouse spleen tissue lysate, mouse epididymis tissue, mouse placenta tissue lysate, rat brain tissue lysate, human liver tissue lysate, human liver tissue, human placenta tissue.
Conjugation
unconjugated
Clone Number
PSH01-52
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IHC-P
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1:1,000
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IF-Tissue
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1:200
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IP
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1-2μg/sample
Target
Function
This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease. atalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia.
Background References
1. Pelletier M.F., Marcil A., Sevigny G., Jakob C.A., Tessier D.C., Chevet E., Menard R., Bergeron J.J.M., Thomas D.Y. The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo. Glycobiology 10:815-827 (2000)
2. Porath B., Gainullin V.G., Cornec-Le Gall E., Dillinger E.K., Heyer C.M., Hopp K., Edwards M.E., Madsen C.D., et al. Mutations in GANAB, encoding the glucosidase IIalpha subunit, cause autosomal-dominant polycystic kidney and liver disease. Am. J. Hum. Genet. 98:1193-1207 (2016)
Subcellular Location
Endoplasmic reticulum, Golgi apparatus.
Synonyms
Alpha glucosidase II alpha subunit antibody
Alpha-glucosidase 2 antibody
G2AN antibody
GANAB antibody
GANAB_HUMAN antibody
Glu II antibody
Glucosidase alpha neutral AB antibody
Glucosidase II alpha antibody
Glucosidase II subunit alpha antibody
GluII antibody
ExpandImages
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Western blot analysis of GANAB on different lysates with Rabbit anti-GANAB antibody (HA750728) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: Raji cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C2C12 cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: 1 minute 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750728) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunofluorescence analysis of paraffin-embedded human thyroid tissue labeling GANAB with Rabbit anti-GANAB antibody (HA750728) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750728, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Western blot analysis of GANAB on different lysates with Rabbit anti-GANAB antibody (HA750728) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: LoVo cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: U-2 OS cell lysate (20 µg/Lane)
Lane 6: K-562 cell lysate (20 µg/Lane)
Lane 7: Jurkat cell lysate (20 µg/Lane)
Lane 8: HL-60 cell lysate (20 µg/Lane)
Lane 9: Raji cell lysate (20 µg/Lane)
Lane 10: NIH/3T3 cell lysate (20 µg/Lane)
Lane 11: C2C12 cell lysate (20 µg/Lane)
Lane 12: RAW264.7 cell lysate (20 µg/Lane)
Lane 13: Mouse spleen tissue lysate (40 µg/Lane)
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750728) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunofluorescence analysis of paraffin-embedded mouse epididymis tissue labeling GANAB with Rabbit anti-GANAB antibody (HA750728) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750728, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Western blot analysis of GANAB on different lysates with Rabbit anti-GANAB antibody (HA750728) at 1/1,000 dilution.
Lane 1: PC-12 cell lysate (20 µg/Lane)
Lane 2: Mouse placenta tissue lysate (40 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Lane 4: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: 3 minutes 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750728) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of GANAB on different lysates with Rabbit anti-GANAB antibody (HA750728) at 1/5,000 dilution.
Lane 1: HeLa-si NT cell lysate
Lane 2: HeLa-si GANAB cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750728) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GANAB antibody (HA750728) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750728) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-GANAB antibody (HA750728) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750728) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-GANAB antibody (HA750728) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750728) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-GANAB antibody (HA750728) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750728) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
GANAB was immunoprecipitated in 0.2mg HeLa cell lysate with HA750728 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750728 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: Rabbit IgG instead of HA750728 in HeLa cell lysate
Lane 3: HA750728 IP in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 21 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"