KDM3A / JHDM2A Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH01-77] (HA750751)
Catalog# HA750751
Rabbit Monoclonal to KDM3A / JHDM2A
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of KDM3A / JHDM2A on different lysates with Rabbit anti-KDM3A / JHDM2A antibody (HA750751) at 1/1,000 dilution.
Lane 1: HCT116 cell lysate
Lane 2: Hela cell lysate
Lane 3: MCF7 cell lysate
Lane 4: Ramos cell lysate
Lane 5: Jurkat cell lysate
Lane 6: NIH/3T3 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 147 kDa
Observed band size: 150 kDa
Exposure time: 2 minutes 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750751) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HCT116 cell lysate
Lane 2: Hela cell lysate
Lane 3: MCF7 cell lysate
Lane 4: Ramos cell lysate
Lane 5: Jurkat cell lysate
Lane 6: NIH/3T3 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 147 kDa
Observed band size: 150 kDa
Exposure time: 2 minutes 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750751) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.