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KDM3A / JHDM2A Recombinant Rabbit Monoclonal Antibody [PSH01-77]

Usd: 385

Specification

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Catalog# HA721729

KDM3A / JHDM2A Recombinant Rabbit Monoclonal Antibody [PSH01-77]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

KDM3A / JHDM2A Recombinant Rabbit Monoclonal Antibody [PSH01-77]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human KDM3A aa 1-400 / 1,321.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 147 kDa

Positive Control

HCT116 cell lysate, Hela cell lysate, MCF7 cell lysate, Ramos cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, human testis tissue, mouse testis tissue.

Conjugation

unconjugated

Clone Number

PSH01-77

RRID

AB_3072841

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

KDM3A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

Background References

1. Yoo J, Jeon YH, Cho HY, et al. Advances in Histone Demethylase KDM3A as a Cancer Therapeutic Target. Cancers (Basel). 2020 Apr 28;12(5):1098.

2. Qian X, Li X, Shi Z, et al. KDM3A Senses Oxygen Availability to Regulate PGC-1α-Mediated Mitochondrial Biogenesis. Mol Cell. 2019 Dec 19;76(6):885-895.

Subcellular Location

Cytoplasm, Nucleus

UNIPROT

SwissProt: Q9Y4C1 Human

SwissProt: Q6PCM1 Mouse

Synonyms

DKFZp686A24246 antibody

DKFZp686P07111 antibody

JHDM2A antibody

JHMD2A antibody

JmjC domain-containing histone demethylation protein 2A antibody

JMJD1 antibody

JMJD1A antibody

Jumonji C domain containing histone demethylase 2A antibody

Jumonji domain containing 1 antibody

Jumonji domain containing 1A antibody

Expand

Images

  • Western blot analysis of KDM3A / JHDM2A on different lysates with Rabbit anti-KDM3A / JHDM2A antibody (<a href="/products/HA721729" style="font-weight: bold;text-decoration: underline;">HA721729</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT116 cell lysate<br />Lane 2: Hela cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: Ramos cell lysate<br />Lane 5: Jurkat cell lysate<br />Lane 6: NIH/3T3 cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 147 kDa<br />Observed band size: 150 kDa<br /><br />Exposure time: 2 minutes 50 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721729" style="font-weight: bold;text-decoration: underline;">HA721729</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of KDM3A / JHDM2A on different lysates with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/1,000 dilution.

    Lane 1: HCT116 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: Ramos cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: NIH/3T3 cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 147 kDa
    Observed band size: 150 kDa

    Exposure time: 2 minutes 50 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721729) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (<a href="/products/HA721729" style="font-weight: bold;text-decoration: underline;">HA721729</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721729" style="font-weight: bold;text-decoration: underline;">HA721729</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721729) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (<a href="/products/HA721729" style="font-weight: bold;text-decoration: underline;">HA721729</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721729" style="font-weight: bold;text-decoration: underline;">HA721729</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-KDM3A / JHDM2A antibody (HA721729) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721729) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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