KMT2C / MLL3 Recombinant Rabbit Monoclonal Antibody [PSH02-11] - BSA and Azide free
Catalog# HA750781
KMT2C / MLL3 Recombinant Rabbit Monoclonal Antibody [PSH02-11] - BSA and Azide free
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WB
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Human
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Rat
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Mouse
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unconjugated
Overview
Product Name
KMT2C / MLL3 Recombinant Rabbit Monoclonal Antibody [PSH02-11] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human KMT2C aa 3,251-3,550 / 4,911.
Species Reactivity
Human, Rat (Predicted: Mouse)
Predicted species support after-sales service
Validated Applications
WB
Target Molecular Weight
Predicted band size: 541 kDa
Positive Control
U-2 OS cell lysate, MCF7 cell lysate, HCT 116 cell lysate, rat skeletal muscle tissue lysate.
Conjugation
unconjugated
Clone Number
PSH02-11
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a nuclear protein with an AT hook DNA-binding domain, a DHHC-type zinc finger, six PHD-type zinc fingers, a SET domain, a post-SET domain and a RING-type zinc finger. This protein is a member of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation.
Background References
1. Na F et al. KMT2C deficiency promotes small cell lung cancer metastasis through DNMT3A-mediated epigenetic reprogramming. Nat Cancer. 2022 Jun
2. Limberger T et al. KMT2C methyltransferase domain regulated INK4A expression suppresses prostate cancer metastasis. Mol Cancer. 2022 Mar
Subcellular Location
Nucleus.
Synonyms
Histone-lysine N-methyltransferase 2C
EC:2.1.1.364
Lysine N-methyltransferase 2C
Homologous to ALR protein
Myeloid/lymphoid or mixed-lineage leukemia protein 3
KMT2C
HALR
KIAA1506
MLL3
Images
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Western blot analysis of KMT2C / MLL3 on different lysates with Rabbit anti-KMT2C / MLL3 antibody (HA750781) at 1/1,000 dilution.
Lane 1: U-2 OS cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (20 µg/Lane)
Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane)
Predicted band size: 541 kDa
Observed band size: 541 kDa
Exposure time: 1 minute 36 seconds;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"