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KMT2C / MLL3 Recombinant Rabbit Monoclonal Antibody [PSH02-11]

Usd: 385

Specification

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Catalog# HA721770

KMT2C / MLL3 Recombinant Rabbit Monoclonal Antibody [PSH02-11]

Application

  • WB

Reactivity

  • Human

  • Rat

BSA and Azide free
Predicted reactivity

  • Mouse

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

KMT2C / MLL3 Recombinant Rabbit Monoclonal Antibody [PSH02-11]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human KMT2C aa 3,251-3,550 / 4,911.

Species Reactivity

Human, Rat (Predicted: Mouse)

Predicted species support after-sales service

Validated Applications

WB

Molecular Weight

Predicted band size: 541 kDa

Positive Control

U-2 OS cell lysate, MCF7 cell lysate, HCT 116 cell lysate, rat skeletal muscle tissue lysate.

Conjugation

unconjugated

Clone Number

PSH02-11

RRID

AB_3072882

Reactivity Data

WB
Human
Tested Tested
Rat
Tested Tested
Mouse
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a nuclear protein with an AT hook DNA-binding domain, a DHHC-type zinc finger, six PHD-type zinc fingers, a SET domain, a post-SET domain and a RING-type zinc finger. This protein is a member of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation.

Background References

1. Na F et al. KMT2C deficiency promotes small cell lung cancer metastasis through DNMT3A-mediated epigenetic reprogramming. Nat Cancer. 2022 Jun

2. Limberger T et al. KMT2C methyltransferase domain regulated INK4A expression suppresses prostate cancer metastasis. Mol Cancer. 2022 Mar

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q8NEZ4 Human

SwissProt: Q8BRH4 Mouse

Entrez Gene: 502710 Rat

Synonyms

Histone-lysine N-methyltransferase 2C

EC:2.1.1.364

Lysine N-methyltransferase 2C

Homologous to ALR protein

Myeloid/lymphoid or mixed-lineage leukemia protein 3

KMT2C

HALR

KIAA1506

MLL3

Images

  • Western blot analysis of KMT2C / MLL3 on different lysates with Rabbit anti-KMT2C / MLL3 antibody (<a href="/products/HA721770" style="font-weight: bold;text-decoration: underline;">HA721770</a>) at 1/1,000 dilution.<br /><br />Lane 1: U-2 OS cell lysate (20 µg/Lane)<br />Lane 2: MCF7 cell lysate (20 µg/Lane)<br />Lane 3: HCT 116 cell lysate (20 µg/Lane)<br />Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 541 kDa<br />Observed band size: 541 kDa<br /><br />Exposure time: 1 minute 36 seconds;<br /><br />3-8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721770" style="font-weight: bold;text-decoration: underline;">HA721770</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of KMT2C / MLL3 on different lysates with Rabbit anti-KMT2C / MLL3 antibody (HA721770) at 1/1,000 dilution.

    Lane 1: U-2 OS cell lysate (20 µg/Lane)
    Lane 2: MCF7 cell lysate (20 µg/Lane)
    Lane 3: HCT 116 cell lysate (20 µg/Lane)
    Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane)

    Predicted band size: 541 kDa
    Observed band size: 541 kDa

    Exposure time: 1 minute 36 seconds;

    3-8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721770) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"