PTGR1 Recombinant Rabbit Monoclonal Antibody [PSH02-12] - BSA and Azide free
Catalog# HA750782
PTGR1 Recombinant Rabbit Monoclonal Antibody [PSH02-12] - BSA and Azide free
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WB
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IHC-P
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FC
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Human
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Rat
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Mouse
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unconjugated
Overview
Product Name
PTGR1 Recombinant Rabbit Monoclonal Antibody [PSH02-12] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PTGR1 aa 1-329 / 329.
Species Reactivity
Human, Rat (Predicted: Mouse)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 36 kDa
Positive Control
HT-29 cell lysate, A549 cell lysate, SiHa cell lysate, A431 cell lysate, rat kidney tissue lysate, rat liver tissue lysate, human small intestine tissue, HeLa.
Conjugation
unconjugated
Clone Number
PSH02-12
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
NAD(P)H-dependent oxidoreductase involved in metabolic inactivation of pro- and anti-inflammatory eicosanoids: prostaglandins (PG), leukotrienes (LT) and lipoxins (LX). atalyzes with high efficiency the reduction of the 13,14 double bond of 15-oxoPGs, including 15-oxo-PGE1, 15-oxo-PGE2, 15-oxo-PGF1-alpha and 15-oxo-PGF2-alpha. Catalyzes with lower efficiency the oxidation of the hydroxyl group at C12 of LTB4 and its derivatives, converting them into biologically less active 12-oxo-LTB4 metabolites. Reduces 15-oxo-LXA4 to 13,14 dihydro-15-oxo-LXA4, enhancing neutrophil recruitment at the inflammatory site. May play a role in metabolic detoxification of alkenals and ketones. Reduces alpha,beta-unsaturated alkenals and ketones, particularly those with medium-chain length, showing highest affinity toward (2E)-decenal and (3E)-3-nonen-2-one (PubMed:25619643). May inactivate 4-hydroxy-2-nonenal, a cytotoxic lipid constituent of oxidized low-density lipoprotein particles.
Background References
1. Ye J et al. Metformin escape in prostate cancer by activating the PTGR1 transcriptional program through a novel super-enhancer. Signal Transduct Target Ther. 2023 Aug
2. Sánchez-Rodríguez R et al. Ptgr1 expression is regulated by NRF2 in rat hepatocarcinogenesis and promotes cell proliferation and resistance to oxidative stress. Free Radic Biol Med. 2017 Jan
Subcellular Location
Cytoplasm.
Synonyms
15-oxoprostaglandin 13-reductase antibody
FLJ99229 antibody
leukotriene B4 12-hydroxydehydrogenase antibody
LTB4DH antibody
MGC34943 antibody
NADP-dependent leukotriene B4 12-hydroxydehydrogenase antibody
PGR1 antibody
PRG-1 antibody
Prostaglandin reductase 1 antibody
Ptgr1 antibody
ExpandImages
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This data was developed using HA721771, the same antibody clone in a different buffer formulation.
Western blot analysis of PTGR1 on different lysates with Rabbit anti-PTGR1 antibody (HA721771) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: SiHa cell lysate (20 µg/Lane)
Lane 4: A431 cell lysate (20 µg/Lane)
Lane 5: Rat kidney tissue lysate (40 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721771) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
This data was developed using HA721771, the same antibody clone in a different buffer formulation.
Western blot analysis of PTGR1 on different lysates with Rabbit anti-PTGR1 antibody (HA721771) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-si-PTGR1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 1 minute 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721771) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721771, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PTGR1 antibody (HA721771) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721771) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721771, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HeLa cells labeling PTGR1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721771, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"