SCXA Recombinant Rabbit Monoclonal Antibody [PSH02-25] - BSA and Azide free
Catalog# HA750793
SCXA Recombinant Rabbit Monoclonal Antibody [PSH02-25] - BSA and Azide free
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
SCXA Recombinant Rabbit Monoclonal Antibody [PSH02-25] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human SCXA aa 1-201 / 201.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 22 kDa
Conjugation
unconjugated
Clone Number
PSH02-25
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
Target
Function
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and bHLH transcription factor binding activity. Contributes to E-box binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleus. Part of transcription regulator complex.
Background References
1. Yang Z et al. SCX-tip-aided LC-MS detection of active ricin via oligonucleotide substrates for depurination kinetics. Analyst. 2023 Jun
2. Ramírez-Aragón M et al. The Transcription Factor SCX is a Potential Serum Biomarker of Fibrotic Diseases. Int J Mol Sci. 2020 Jul
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Basic helix loop helix transcription factor scleraxis antibody
Basic helix-loop-helix transcription factor scleraxis antibody
bHLHa41 antibody
bHLHa48 antibody
Class A basic helix-loop-helix protein 41 antibody
Class A basic helix-loop-helix protein 48 antibody
scleraxis antibody
scleraxis homolog A antibody
SCX antibody
SCX_HUMAN antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of SCXA on different lysates with Rabbit anti-SCXA antibody (HA750793) at 1/1,000 dilution.
Lane 1: HEK-293-NT cell lysate
Lane 2: HEK-293-OE-SCXA cell lysate
Lane 3: HeLa-NT cell lysate
Lane 4: HeLa-OE-SCXA cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 27 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750793) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of HeLa transfected with or without SCXA cells labeling SCXA with Rabbit anti-SCXA antibody (HA750793) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCXA antibody (HA750793) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"