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SCXA Recombinant Rabbit Monoclonal Antibody [PSH02-25]

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Specification

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Catalog# HA721805

SCXA Recombinant Rabbit Monoclonal Antibody [PSH02-25]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

SCXA Recombinant Rabbit Monoclonal Antibody [PSH02-25]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SCXA aa 1-201 / 201.

Species Reactivity

Human

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 22 kDa

Conjugation

unconjugated

Clone Number

PSH02-25

RRID

AB_3072917

Reactivity Data

WB IF-Cell
Human
Tested Tested
Tested Tested
Mouse
Rat

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

Enables DNA-binding transcription activator activity, RNA polymerase II-specific and bHLH transcription factor binding activity. Contributes to E-box binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleus. Part of transcription regulator complex.

Background References

1. Yang Z et al. SCX-tip-aided LC-MS detection of active ricin via oligonucleotide substrates for depurination kinetics. Analyst. 2023 Jun

2. Ramírez-Aragón M et al. The Transcription Factor SCX is a Potential Serum Biomarker of Fibrotic Diseases. Int J Mol Sci. 2020 Jul

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q7RTU7 Human

Synonyms

Basic helix loop helix transcription factor scleraxis antibody

Basic helix-loop-helix transcription factor scleraxis antibody

bHLHa41 antibody

bHLHa48 antibody

Class A basic helix-loop-helix protein 41 antibody

Class A basic helix-loop-helix protein 48 antibody

scleraxis antibody

scleraxis homolog A antibody

SCX antibody

SCX_HUMAN antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of SCXA on different lysates with Rabbit anti-SCXA antibody (<a href="/products/HA721805" style="font-weight: bold;text-decoration: underline;">HA721805</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293-NT cell lysate<br />Lane 2: HEK-293-OE-SCXA cell lysate<br />Lane 3: HeLa-NT cell lysate<br />Lane 4: HeLa-OE-SCXA cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 22 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 24 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721805" style="font-weight: bold;text-decoration: underline;">HA721805</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of SCXA on different lysates with Rabbit anti-SCXA antibody (HA721805) at 1/1,000 dilution.

    Lane 1: HEK-293-NT cell lysate
    Lane 2: HEK-293-OE-SCXA cell lysate
    Lane 3: HeLa-NT cell lysate
    Lane 4: HeLa-OE-SCXA cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 22 kDa
    Observed band size: 27 kDa

    Exposure time: 24 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721805) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunocytochemistry analysis of HeLa transfected with or without SCXA cells labeling SCXA with Rabbit anti-SCXA antibody (<a href="/products/HA721805" style="font-weight: bold;text-decoration: underline;">HA721805</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCXA antibody (<a href="/products/HA721805" style="font-weight: bold;text-decoration: underline;">HA721805</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Cell treatment (CT)

    Immunocytochemistry analysis of HeLa transfected with or without SCXA cells labeling SCXA with Rabbit anti-SCXA antibody (HA721805) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCXA antibody (HA721805) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"