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ATG4B Recombinant Rabbit Monoclonal Antibody [PSH02-53] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750816

ATG4B Recombinant Rabbit Monoclonal Antibody [PSH02-53] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ATG4B Recombinant Rabbit Monoclonal Antibody [PSH02-53] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ATG4B aa 1-393 / 393 (Q9Y4P1).

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB

Molecular Weight

Predicted band size: 44 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, 293T cell lysate, MCF7 cell lysate, K-562 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Mouse kidney tissue lysate, Mouse colon tissue lysate.

Conjugation

unconjugated

Clone Number

PSH02-53

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Cysteine protease ATG4B is an enzyme that in humans is encoded by the ATG4B gene. Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. One main function of Atg4 is to cleave the pre-protein of Atg8, leading to the non-lipidated soluble (-I) form which can be processed further by Atg3, Atg7, Atg5-12 into the lipidated form (-II) anchored to the autophagic membrane.

Background References

1. Sun L et al. Deacetylation of ATG4B promotes autophagy initiation under starvation. Sci Adv. 2022 Aug

2. Yang G et al. Targeting Atg4B for cancer therapy: Chemical mediators. Eur J Med Chem. 2021 Jan

Subcellular Location

Cytoplasm, cytosol, Cytoplasmic vesicle, autophagosome, Endoplasmic reticulum, Mitochondrion.

UNIPROT

SwissProt: Q9Y4P1 Human

SwissProt: Q8BGE6 Mouse

SwissProt: Q62625 Rat

Synonyms

APG4 autophagy 4 homolog B antibody

APG4B antibody

ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody

ATG4B antibody

ATG4B_HUMAN antibody

AUT like 1 cysteine endopeptidase antibody

AUT-like 1 cysteine endopeptidase antibody

AUTL1 antibody

Autophagin 1 antibody

Autophagin-1 antibody

Expand

Images

  • Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (<a href="/products/HA750816" style="font-weight: bold;text-decoration: underline;">HA750816</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: 293T cell lysate<br />Lane 4: MCF7 cell lysate<br />Lane 5: K-562 cell lysate<br />Lane 6: COS-1 cell lysate<br />Lane 7: NIH/3T3 cell lysate<br />Lane 8: C2C12 cell lysate<br />Lane 9: PC-12 cell lysate<br />Lane 10: Mouse brain tissue lysate<br />Lane 11: Rat brain tissue lysate<br />Lane 12: Mouse kidney tissue lysate<br />Lane 13: Mouse colon tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 44 kDa<br />Observed band size: 47 kDa<br /><br />Exposure time: 42 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750816" style="font-weight: bold;text-decoration: underline;">HA750816</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (HA750816) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: 293T cell lysate
    Lane 4: MCF7 cell lysate
    Lane 5: K-562 cell lysate
    Lane 6: COS-1 cell lysate
    Lane 7: NIH/3T3 cell lysate
    Lane 8: C2C12 cell lysate
    Lane 9: PC-12 cell lysate
    Lane 10: Mouse brain tissue lysate
    Lane 11: Rat brain tissue lysate
    Lane 12: Mouse kidney tissue lysate
    Lane 13: Mouse colon tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 44 kDa
    Observed band size: 47 kDa

    Exposure time: 42 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750816) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (<a href="/products/HA750816" style="font-weight: bold;text-decoration: underline;">HA750816</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate (20 µg/Lane)<br />Lane 2: MCF7 cell lysate (20 µg/Lane)<br />Lane 3: COS-1 cell lysate (20 µg/Lane)<br />Lane 4: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 5: PC-12 cell lysate (20 µg/Lane)<br />Lane 6: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 7: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 44 kDa<br />Observed band size: 47 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750816" style="font-weight: bold;text-decoration: underline;">HA750816</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (HA750816) at 1/2,000 dilution.

    Lane 1: 293T cell lysate (20 µg/Lane)
    Lane 2: MCF7 cell lysate (20 µg/Lane)
    Lane 3: COS-1 cell lysate (20 µg/Lane)
    Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 5: PC-12 cell lysate (20 µg/Lane)
    Lane 6: Mouse brain tissue lysate (40 µg/Lane)
    Lane 7: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 44 kDa
    Observed band size: 47 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750816) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (<a href="/products/HA750816" style="font-weight: bold;text-decoration: underline;">HA750816</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T-si NT cell lysate<br />Lane 2: 293T-si ATG4B cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 44 kDa<br />Observed band size: 47 kDa<br /><br />Exposure time: 51 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750816" style="font-weight: bold;text-decoration: underline;">HA750816</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (HA750816) at 1/1,000 dilution.

    Lane 1: 293T-si NT cell lysate
    Lane 2: 293T-si ATG4B cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 44 kDa
    Observed band size: 47 kDa

    Exposure time: 51 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750816) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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