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RHOG Recombinant Rabbit Monoclonal Antibody [PSH02-70] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750827

RHOG Recombinant Rabbit Monoclonal Antibody [PSH02-70] - BSA and Azide free

Application

  • WB

  • FC

Reactivity

  • Human

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

RHOG Recombinant Rabbit Monoclonal Antibody [PSH02-70] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human RHOG aa 1-50 / 191.

Species Reactivity

Human, Green monkey

Validated Applications

WB, FC

Target Molecular Weight

Predicted band size: 21 kDa

Positive Control

Jurkat cell lysate, MDA-MB-231 cell lysate, K-562 cell lysate, Hela cell lysate, MCF7 cell lysate, PANC-1 cell lysate, COS-1 cell lysate, HeLa.

Conjugation

unconjugated

Clone Number

PSH02-70

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Plays a role in immunological synaptic F-actin density and architecture organization. Regulates actin reorganization in lymphocytes, possibly through the modulation of Rac1 activity. Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. Binds phospholipids in an activation-dependent manner; thereby acting as an anchor for other proteins to the plasma membrane (PM). Plays a role in exocytosis of cytotoxic granules (CG) by lymphocytes/Component of the exocytosis machinery in natural killer (NK) and CD8+ T cells. Promotes the docking of cytotoxic granules (CG) to the plasma membrane through the interaction with UNC13D. Involved in the cytotoxic activity of lymphocytes/primary CD8+ T cells.

Background References

1. Kalinichenko A, Perinetti Casoni G, Dupré L, et al. RhoG deficiency abrogates cytotoxicity of human lymphocytes and causes hemophagocytic lymphohistiocytosis. Blood. 2021 Apr 15;137(15):2033-2045.

2. Ahmad Mokhtar AM, Salikin NH, Haron AS, et al. RhoG\'s Role in T Cell Activation and Function. Front Immunol. 2022 Feb 25;13:845064.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt:P84095 Human

SwissProt:A0A0D9QW62 Green monkey

Synonyms

ARHG antibody

Ras homolog family member G antibody

Rho G antibody

Rho related GTP binding protein RhoG precursor antibody

Rho-related GTP-binding protein RhoG antibody

RHOG antibody

RHOG_HUMAN antibody

Images

  • This data was developed using <a href="/products/HA721848" style="font-weight: bold;text-decoration: underline;">HA721848</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of RHOG on different lysates with Rabbit anti-RHOG antibody (<a href="/products/HA721848" style="font-weight: bold;text-decoration: underline;">HA721848</a>) at 1/2,000 dilution.<br /><br />Lane 1: Jurkat cell lysate (20 µg/Lane)<br />Lane 2: MDA-MB-231 cell lysate (20 µg/Lane)<br />Lane 3: K-562 cell lysate (20 µg/Lane)<br />Lane 4: Hela cell lysate (20 µg/Lane)<br />Lane 5: MCF7 cell lysate (20 µg/Lane)<br />Lane 6: PANC-1 cell lysate (20 µg/Lane)<br />Lane 7: COS-1 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa<br /><br />Exposure time: 30 seconds; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721848" style="font-weight: bold;text-decoration: underline;">HA721848</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA721848, the same antibody clone in a different buffer formulation.
    Western blot analysis of RHOG on different lysates with Rabbit anti-RHOG antibody (HA721848) at 1/2,000 dilution.

    Lane 1: Jurkat cell lysate (20 µg/Lane)
    Lane 2: MDA-MB-231 cell lysate (20 µg/Lane)
    Lane 3: K-562 cell lysate (20 µg/Lane)
    Lane 4: Hela cell lysate (20 µg/Lane)
    Lane 5: MCF7 cell lysate (20 µg/Lane)
    Lane 6: PANC-1 cell lysate (20 µg/Lane)
    Lane 7: COS-1 cell lysate (20 µg/Lane)

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

    Exposure time: 30 seconds; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721848) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA721848" style="font-weight: bold;text-decoration: underline;">HA721848</a>, the same antibody clone in a different buffer formulation.<br />Flow cytometric analysis of HeLa cells labeling RHOG.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721848" style="font-weight: bold;text-decoration: underline;">HA721848</a>, 1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    This data was developed using HA721848, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of HeLa cells labeling RHOG.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721848, 1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"