RHOG Recombinant Rabbit Monoclonal Antibody [PSH02-70]
Catalog# HA721848
RHOG Recombinant Rabbit Monoclonal Antibody [PSH02-70]
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WB
-
FC
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Human
-
Green monkey
-
unconjugated
Overview
Product Name
RHOG Recombinant Rabbit Monoclonal Antibody [PSH02-70]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human RHOG aa 1-50 / 191.
Species Reactivity
Human, Green monkey
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 21 kDa
Positive Control
Jurkat cell lysate, MDA-MB-231 cell lysate, K-562 cell lysate, Hela cell lysate, MCF7 cell lysate, PANC-1 cell lysate, COS-1 cell lysate, HeLa.
Conjugation
unconjugated
Clone Number
PSH02-70
RRID
Reactivity Data
| WB | FC | |
|---|---|---|
| Human |
|
|
| Green Monkey |
|
|
| Mouse |
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
FC
-
1:1,000
Target
Function
Plays a role in immunological synaptic F-actin density and architecture organization. Regulates actin reorganization in lymphocytes, possibly through the modulation of Rac1 activity. Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. Binds phospholipids in an activation-dependent manner; thereby acting as an anchor for other proteins to the plasma membrane (PM). Plays a role in exocytosis of cytotoxic granules (CG) by lymphocytes/Component of the exocytosis machinery in natural killer (NK) and CD8+ T cells. Promotes the docking of cytotoxic granules (CG) to the plasma membrane through the interaction with UNC13D. Involved in the cytotoxic activity of lymphocytes/primary CD8+ T cells.
Background References
1. Kalinichenko A, Perinetti Casoni G, Dupré L, et al. RhoG deficiency abrogates cytotoxicity of human lymphocytes and causes hemophagocytic lymphohistiocytosis. Blood. 2021 Apr 15;137(15):2033-2045.
2. Ahmad Mokhtar AM, Salikin NH, Haron AS, et al. RhoG\'s Role in T Cell Activation and Function. Front Immunol. 2022 Feb 25;13:845064.
Subcellular Location
Cell membrane.
Synonyms
ARHG antibody
Ras homolog family member G antibody
Rho G antibody
Rho related GTP binding protein RhoG precursor antibody
Rho-related GTP-binding protein RhoG antibody
RHOG antibody
RHOG_HUMAN antibody
Images
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Western blot analysis of RHOG on different lysates with Rabbit anti-RHOG antibody (HA721848) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: Hela cell lysate (20 µg/Lane)
Lane 5: MCF7 cell lysate (20 µg/Lane)
Lane 6: PANC-1 cell lysate (20 µg/Lane)
Lane 7: COS-1 cell lysate (20 µg/Lane)
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721848) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of HeLa cells labeling RHOG.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721848, 1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"