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CD58 Recombinant Rabbit Monoclonal Antibody [PSH02-74] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750829

CD58 Recombinant Rabbit Monoclonal Antibody [PSH02-74] - BSA and Azide free

Application

  • WB

  • FC

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CD58 Recombinant Rabbit Monoclonal Antibody [PSH02-74] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CD58 aa 29-215 (Extracellular).

Species Reactivity

Human

Validated Applications

WB, FC

Target Molecular Weight

Predicted band size: 28 kDa

Positive Control

HeLa cell lysate, Raji cell lysate, Jurkat cell lysate, K-562 cell lysate, HUVEC cell lysate, HT-29 cell lysate, human peripheral blood lymphocytes.

Conjugation

unconjugated

Clone Number

PSH02-74

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.

Background References

1. Zhang Y et al. CD58 Immunobiology at a Glance. Front Immunol. 2021 Jun

2. Yan X et al. CD58 loss in tumor cells confers functional impairment of CAR T cells. Blood Adv. 2022 Nov

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P19256 Human

Synonyms

AG3 antibody

CD58 antibody

CD58 antigen (lymphocyte function associated antigen 3)1 antibody

CD58 antigen antibody

CD58 antigen, (lymphocyte function associated antigen 3) antibody

CD58 molecule antibody

FLJ23181 antibody

FLJ43722 antibody

LFA 3 antibody

LFA-3 antibody

Expand

Images

  • Western blot analysis of CD58 on different lysates with Rabbit anti-CD58 antibody (<a href="/products/HA750829" style="font-weight: bold;text-decoration: underline;">HA750829</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Raji cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: HUVEC cell lysate<br />Lane 6: HT-29 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 5 minutes; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA750829" style="font-weight: bold;text-decoration: underline;">HA750829</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 28 kDa<br />Observed band size: 55 kDa

    Western blot analysis of CD58 on different lysates with Rabbit anti-CD58 antibody (HA750829) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Raji cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: HUVEC cell lysate
    Lane 6: HT-29 cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 5 minutes; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA750829, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 28 kDa
    Observed band size: 55 kDa

  • Flow cytometric analysis of human peripheral blood lymphocytes labeling CD58.<br /><br />Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (<a href="/products/HA750829" style="font-weight: bold;text-decoration: underline;">HA750829</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (black). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃.

    Flow cytometric analysis of human peripheral blood lymphocytes labeling CD58.

    Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA750829, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (black). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

CD58 Recombinant Rabbit Monoclonal Antibody [PSH02-74]

Application: WB,FC

Reactivity: Human

Conjugate: unconjugated