CD58 Recombinant Rabbit Monoclonal Antibody [PSH02-74]
Catalog# HA721852
CD58 Recombinant Rabbit Monoclonal Antibody [PSH02-74]
-
WB
-
FC
-
Human
-
unconjugated
Overview
Product Name
CD58 Recombinant Rabbit Monoclonal Antibody [PSH02-74]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CD58 aa 29-215 (Extracellular).
Species Reactivity
Human
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 28 kDa
Positive Control
HeLa cell lysate, Raji cell lysate, Jurkat cell lysate, K-562 cell lysate, HUVEC cell lysate, HT-29 cell lysate, human peripheral blood lymphocytes.
Conjugation
unconjugated
Clone Number
PSH02-74
RRID
Reactivity Data
| WB | FC | |
|---|---|---|
| Human |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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FC
-
1:1,000
Target
Function
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.
Background References
1. Zhang Y et al. CD58 Immunobiology at a Glance. Front Immunol. 2021 Jun
2. Yan X et al. CD58 loss in tumor cells confers functional impairment of CAR T cells. Blood Adv. 2022 Nov
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
AG3 antibody
CD58 antibody
CD58 antigen (lymphocyte function associated antigen 3)1 antibody
CD58 antigen antibody
CD58 antigen, (lymphocyte function associated antigen 3) antibody
CD58 molecule antibody
FLJ23181 antibody
FLJ43722 antibody
LFA 3 antibody
LFA-3 antibody
ExpandImages
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Western blot analysis of CD58 on different lysates with Rabbit anti-CD58 antibody (HA721852) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Raji cell lysate
Lane 3: Jurkat cell lysate
Lane 4: K-562 cell lysate
Lane 5: HUVEC cell lysate
Lane 6: HT-29 cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 5 minutes; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721852, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 28 kDa
Observed band size: 55 kDa -
Flow cytometric analysis of human peripheral blood lymphocytes labeling CD58.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721852, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (black). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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