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GALT Recombinant Rabbit Monoclonal Antibody [PSH02-88] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750842

GALT Recombinant Rabbit Monoclonal Antibody [PSH02-88] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

GALT Recombinant Rabbit Monoclonal Antibody [PSH02-88] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human GALT aa 1-379 / 379.

Species Reactivity

Human, Mouse

Validated Applications

WB

Target Molecular Weight

Predicted band size: 43 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, A549 cell lysate, K-562 cell lysate, THP-1 cell lysate, Jurkat cell lysate, SiHa cell lysate, SH-SY5Y cell lysate, mouse liver tissue lysate.

Conjugation

unconjugated

Clone Number

PSH02-88

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

Target

Function

Galactose-1-phosphate uridylyltransferase (or GALT, G1PUT) is an enzyme responsible for converting ingested galactose to glucose. Deficiency of GALT causes classic galactosemia. Galactosemia is an autosomal recessive inherited disorder detectable in newborns and childhood. Classical galactosemia (G/G) is caused by a deficiency in GALT activity, whereas the more common clinical manifestations, Duarte (D/D) and the Duarte/Classical variant (D/G) are caused by the attenuation of GALT activity. Symptoms include ovarian failure, developmental coordination disorder (difficulty speaking correctly and consistently), and neurologic deficits. A single mutation in any of several base pairs can lead to deficiency in GALT activity. Screening has mostly eliminated neonatal death by G/G galactosemia, but the disease, due to GALT’s role in the biochemical metabolism of ingested galactose (which is toxic when accumulated) to the energetically useful glucose, can certainly be fatal. However, those afflicted with galactosemia can live relatively normal lives by avoiding milk products and anything else containing galactose (because it cannot be metabolized), but there is still the potential for problems in neurological development or other complications, even in those who avoid galactose.

Background References

1. Latchman K. et. al. A founder noncoding GALT variant interfering with splicing causes galactosemia. J Inherit Metab Dis. 2020 Nov

2. Mörbe UM. et. al. Human gut-associated lymphoid tissues (GALT); diversity, structure, and function. Mucosal Immunol. 2021 Jul

Subcellular Location

Cytosol, Golgi apparatus, cytoplasm.

UNIPROT

SwissProt: P07902 Human

SwissProt: Q03249 Mouse

Synonyms

Gal 1 P uridylyltransferase antibody

Gal-1-P uridylyltransferase antibody

Galactose 1 phosphate uridyl transferase antibody

Galactose 1 phosphate uridylyltransferase antibody

Galactose-1-phosphate uridylyltransferase antibody

GALT antibody

GALT_HUMAN antibody

UDP glucose hexose 1 phosphate uridylyltransferase antibody

UDP-glucose--hexose-1-phosphate uridylyltransferase antibody

Images

  • Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (<a href="/products/HA750842" style="font-weight: bold;text-decoration: underline;">HA750842</a>) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (15 µg/Lane)<br />Lane 2: HeLa cell lysate (15 µg/Lane)<br />Lane 3: A549 cell lysate (15 µg/Lane)<br />Lane 4: K-562 cell lysate (15 µg/Lane)<br />Lane 5: THP-1 cell lysate (15 µg/Lane)<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: Lane 1-5 (left): 1 minute 34 seconds; ECL: K1801; <br />Exposure time: Lane 1-5 (right): 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750842" style="font-weight: bold;text-decoration: underline;">HA750842</a>) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA750842) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate (15 µg/Lane)
    Lane 2: HeLa cell lysate (15 µg/Lane)
    Lane 3: A549 cell lysate (15 µg/Lane)
    Lane 4: K-562 cell lysate (15 µg/Lane)
    Lane 5: THP-1 cell lysate (15 µg/Lane)

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

    Exposure time: Lane 1-5 (left): 1 minute 34 seconds; ECL: K1801;
    Exposure time: Lane 1-5 (right): 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750842) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (<a href="/products/HA750842" style="font-weight: bold;text-decoration: underline;">HA750842</a>) at 1/5,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-GALT KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 46 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750842" style="font-weight: bold;text-decoration: underline;">HA750842</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA750842) at 1/5,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-GALT KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

    Exposure time: 46 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750842) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (<a href="/products/HA750842" style="font-weight: bold;text-decoration: underline;">HA750842</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: A549 cell lysate (20 µg/Lane)<br />Lane 4: K-562 cell lysate (20 µg/Lane)<br />Lane 5: THP-1 cell lysate (20 µg/Lane)<br />Lane 6: Jurkat cell lysate (20 µg/Lane)<br />Lane 7: SiHa cell lysate (20 µg/Lane)<br />Lane 8: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 9: Mouse liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 43 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750842" style="font-weight: bold;text-decoration: underline;">HA750842</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA750842) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: A549 cell lysate (20 µg/Lane)
    Lane 4: K-562 cell lysate (20 µg/Lane)
    Lane 5: THP-1 cell lysate (20 µg/Lane)
    Lane 6: Jurkat cell lysate (20 µg/Lane)
    Lane 7: SiHa cell lysate (20 µg/Lane)
    Lane 8: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 9: Mouse liver tissue lysate (40 µg/Lane)

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

    Exposure time: 43 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750842) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"