Wee1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH05-11] (HA750984)
Catalog# HA750984
Rabbit Monoclonal to Wee1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Wee1 on different lysates with Rabbit anti-Wee1 antibody (HA750984) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Raji cell lysate
Lane 3: 293T cell lysate
Lane 4: HT-29 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: COS-1 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 90 kDa
Exposure time: 43 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750984) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: Raji cell lysate
Lane 3: 293T cell lysate
Lane 4: HT-29 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: COS-1 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 90 kDa
Exposure time: 43 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750984) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.