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Wee1 Recombinant Rabbit Monoclonal Antibody [PSH05-11]

Usd: 385

Specification

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Catalog# HA722227

Wee1 Recombinant Rabbit Monoclonal Antibody [PSH05-11]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Wee1 Recombinant Rabbit Monoclonal Antibody [PSH05-11]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Wee1 aa 251-600 / 646.

Species Reactivity

Human, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 72 kDa

Positive Control

HeLa cell lysate, Raji cell lysate, 293T cell lysate, HT-29 cell lysate, Jurkat cell lysate, COS-1 cell lysate, human testis tissue.

Conjugation

unconjugated

Clone Number

PSH05-11

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

Target

Function

Wee1 is a nuclear kinase belonging to the Ser/Thr family of protein kinases in the fission yeast Schizosaccharomyces pombe (S. pombe). Wee1 has a molecular mass of 96 kDa and is a key regulator of cell cycle progression. It influences cell size by inhibiting the entry into mitosis, through inhibiting Cdk1. Wee1 has homologues in many other organisms, including mammals. The regulation of cell size is critical to ensure functionality of a cell. Besides environmental factors such as nutrients, growth factors and functional load, cell size is also controlled by a cellular cell size checkpoint. Wee1 is a component of this checkpoint. It is a kinase determining the timepoint of entry into mitosis, thus influencing the size of the daughter cells. Loss of Wee1 function will produce smaller than normal daughter cell, because cell division occurs prematurely.

Background References

1. Kong A et al. WEE1 Inhibitor: Clinical Development. Curr Oncol Rep. 2021 Jul

2. Taniguchi H et al. WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell Rep. 2022 May

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P30291 Human

Synonyms

DKFZp686I18166 antibody

EC 2.7.10.2 antibody

FLJ16446 antibody

MGC105683 antibody

OTTHUMP00000231338 antibody

OTTHUMP00000231339 antibody

Wee 1 antibody

WEE 1 homolog 1 (S. pombe) antibody

WEE1 antibody

WEE1 homolog (S. pombe) antibody

Expand

Images

  • Western blot analysis of Wee1 on different lysates with Rabbit anti-Wee1 antibody (<a href="/products/HA722227" style="font-weight: bold;text-decoration: underline;">HA722227</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Raji cell lysate<br />Lane 3: 293T cell lysate<br />Lane 4: HT-29 cell lysate<br />Lane 5: Jurkat cell lysate<br />Lane 6: COS-1 cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 72 kDa<br />Observed band size: 90 kDa<br /><br />Exposure time: 43 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722227" style="font-weight: bold;text-decoration: underline;">HA722227</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Wee1 on different lysates with Rabbit anti-Wee1 antibody (HA722227) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Raji cell lysate
    Lane 3: 293T cell lysate
    Lane 4: HT-29 cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: COS-1 cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 72 kDa
    Observed band size: 90 kDa

    Exposure time: 43 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722227) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Wee1 antibody (<a href="/products/HA722227" style="font-weight: bold;text-decoration: underline;">HA722227</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722227" style="font-weight: bold;text-decoration: underline;">HA722227</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Wee1 antibody (HA722227) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722227) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation