ZW10 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH05-80] (HA751028)
Catalog# HA751028
Rabbit Monoclonal to ZW10
Tested for
-
WB
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of ZW10 on different lysates with Rabbit anti-ZW10 antibody (HA751028) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lane 3: Ramos cell lysate
Lane 4: U-2 OS cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: SW620 cell lysate
Lane 7: HeLa cell lysate
Lane 8: COS-1 cell lysate
Lane 9: NIH/3T3 cell lysate
Lane 10: C6 cell lysate
Lane 11: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751028) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lane 3: Ramos cell lysate
Lane 4: U-2 OS cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: SW620 cell lysate
Lane 7: HeLa cell lysate
Lane 8: COS-1 cell lysate
Lane 9: NIH/3T3 cell lysate
Lane 10: C6 cell lysate
Lane 11: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751028) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.