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ZW10 Recombinant Rabbit Monoclonal Antibody [PSH05-80]

Usd: 385

Specification

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Catalog# HA722479

ZW10 Recombinant Rabbit Monoclonal Antibody [PSH05-80]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

ZW10 Recombinant Rabbit Monoclonal Antibody [PSH05-80]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human ZW10 aa 350-779.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 89 kDa

Positive Control

Jurkat cell lysate, K-562 cell lysate, Ramos cell lysate, U-2 OS cell lysate, HCT 116 cell lysate, SW620 cell lysate, HeLa cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, human testis tissue, mouse testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

PSH05-80

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:2,000

Target

Function

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER. Overexpression as well as silencing of ZW10 disrupts the morphology of the ER-Golgi intermediate compartment as well as the Golgi apparatus and slows down ER-Golgi transport.

Background References

1. Hirose H., Arasaki K., Dohmae N., Takio K., Hatsuzawa K., Nagahama M., Tani K., Yamamoto A., Tohyama M., Tagaya M. Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi. EMBO J. 23:1267-1278 (2004)

2. Scaeerou F., Starr D.A., Piano F., Papoulas O., Karess R.E., Goldberg M.L. The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore. J. Cell Sci. 114:3103-3114 (2001)

Subcellular Location

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Endoplasmic reticulum.

UNIPROT

SwissProt: O43264 Human

SwissProt: O54692 Mouse

SwissProt: Q4V8C2 Rat

Synonyms

Centromere/kinetochore protein zw10 antibody

Centromere/kinetochore protein zw10 homolog antibody

HZW 10 antibody

HZW10 antibody

Kinetochore associated homolog antibody

KNTC1AP antibody

MGC149821 antibody

Zeste white 10 antibody

Zeste white 10 homolog antibody

ZW 10 antibody

Expand

Images

  • Western blot analysis of ZW10 on different lysates with Rabbit anti-ZW10 antibody (<a href="/products/HA722479" style="font-weight: bold;text-decoration: underline;">HA722479</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: K-562 cell lysate<br />Lane 3: Ramos cell lysate<br />Lane 4: U-2 OS cell lysate<br />Lane 5: HCT 116 cell lysate<br />Lane 6: SW620 cell lysate<br />Lane 7: HeLa cell lysate<br />Lane 8: COS-1 cell lysate<br />Lane 9: NIH/3T3 cell lysate<br />Lane 10: C6 cell lysate<br />Lane 11: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 89 kDa<br />Observed band size: 89 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722479" style="font-weight: bold;text-decoration: underline;">HA722479</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ZW10 on different lysates with Rabbit anti-ZW10 antibody (HA722479) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: K-562 cell lysate
    Lane 3: Ramos cell lysate
    Lane 4: U-2 OS cell lysate
    Lane 5: HCT 116 cell lysate
    Lane 6: SW620 cell lysate
    Lane 7: HeLa cell lysate
    Lane 8: COS-1 cell lysate
    Lane 9: NIH/3T3 cell lysate
    Lane 10: C6 cell lysate
    Lane 11: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 89 kDa
    Observed band size: 89 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722479) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ZW10 antibody (<a href="/products/HA722479" style="font-weight: bold;text-decoration: underline;">HA722479</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722479" style="font-weight: bold;text-decoration: underline;">HA722479</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ZW10 antibody (HA722479) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722479) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-ZW10 antibody (<a href="/products/HA722479" style="font-weight: bold;text-decoration: underline;">HA722479</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722479" style="font-weight: bold;text-decoration: underline;">HA722479</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-ZW10 antibody (HA722479) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722479) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ZW10 antibody (<a href="/products/HA722479" style="font-weight: bold;text-decoration: underline;">HA722479</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722479" style="font-weight: bold;text-decoration: underline;">HA722479</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ZW10 antibody (HA722479) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722479) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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