AIDA Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH05-81] (HA751029)
Catalog# HA751029
Rabbit Monoclonal to AIDA
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of AIDA on different lysates with Rabbit anti-AIDA antibody (HA751029) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: Jurkat cell lysate
Lane 4: 293T cell lysate
Lane 5: MEF cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: C2C12 cell lysate
Lane 9: Neuro-2a cell lysate
Lane 10: PC-12 cell lysate
Lane 11: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time (left): 3 minutes; ECL: K1801;
Exposure time (right): 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751029) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: Jurkat cell lysate
Lane 4: 293T cell lysate
Lane 5: MEF cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: C2C12 cell lysate
Lane 9: Neuro-2a cell lysate
Lane 10: PC-12 cell lysate
Lane 11: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time (left): 3 minutes; ECL: K1801;
Exposure time (right): 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751029) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.