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AIDA Recombinant Rabbit Monoclonal Antibody [PSH05-81]

Usd: 385

Specification

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Catalog# HA722480

AIDA Recombinant Rabbit Monoclonal Antibody [PSH05-81]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

AIDA Recombinant Rabbit Monoclonal Antibody [PSH05-81]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human AIDA aa 1-306.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 35 kDa

Positive Control

HeLa cell lysate, U-87 MG cell lysate, Jurkat cell lysate, 293T cell lysate, MEF cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, C6 cell lysate.

Conjugation

unconjugated

Clone Number

PSH05-81

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling. Predicted to enable phosphatidylinositol binding activity. Acts upstream of or within negative regulation of JUN kinase activity. Predicted to be located in cytoplasm. Predicted to be active in membrane.

Background References

1. Lalonde S., Codina-Fauteux V.A., de Bellefon S.M., Leblanc F., Beaudoin M., Simon M.M., Dali R., Kwan T., Lo K.S., Lettre G. Integrative analysis of vascular endothelial cell genomic features identifies AIDA as a coronary artery disease candidate gene. Genome Biol 20:133-133 (2019)

2. Zhang P., Luo J., Wu T., Wang X., Yang F., Yu Y., Lu L., Yu H. MiR-32-5p/AIDA Mediates OxLDL-Induced Endothelial Injury and Inflammation. Int Heart J 63:928-938 (2022)

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q96BJ3 Human

SwissProt: Q8C4Q6 Mouse

Entrez Gene: 682999 Rat

Synonyms

2610208M17Rik antibody

AIDA antibody

AIDA_HUMAN antibody

Axin interaction partner and dorsalization antagonist antibody

Axin interactor antibody

Axin interactor dorsalization associated protein antibody

Axin interactor, dorsalization associated antibody

C1orf80 antibody

Chromosome 1 open reading frame 80 antibody

dorsalization-associated protein antibody

Expand

Images

  • Western blot analysis of AIDA on different lysates with Rabbit anti-AIDA antibody (<a href="/products/HA722480" style="font-weight: bold;text-decoration: underline;">HA722480</a>) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: U-87 MG cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: 293T cell lysate<br />Lane 5: MEF cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: RAW264.7 cell lysate<br />Lane 8: C2C12 cell lysate<br />Lane 9: Neuro-2a cell lysate<br />Lane 10: PC-12 cell lysate<br />Lane 11: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time (left): 3 minutes; ECL: K1801;<br />Exposure time (right): 59 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722480" style="font-weight: bold;text-decoration: underline;">HA722480</a>) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AIDA on different lysates with Rabbit anti-AIDA antibody (HA722480) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: U-87 MG cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: 293T cell lysate
    Lane 5: MEF cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: RAW264.7 cell lysate
    Lane 8: C2C12 cell lysate
    Lane 9: Neuro-2a cell lysate
    Lane 10: PC-12 cell lysate
    Lane 11: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    Exposure time (left): 3 minutes; ECL: K1801;
    Exposure time (right): 59 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722480) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"