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beta Amyloid 1-40 Recombinant Rabbit Monoclonal Antibody [PSH05-86] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751034

beta Amyloid 1-40 Recombinant Rabbit Monoclonal Antibody [PSH05-86] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

beta Amyloid 1-40 Recombinant Rabbit Monoclonal Antibody [PSH05-86] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human beta Amyloid 1-40 peptide.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 4 kDa

Positive Control

APP/PS1, 6-month mouse of AD brain tissue.

Conjugation

unconjugated

Clone Number

PSH05-86

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:200

Target

Function

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.

Background References

1. Janelidze S et al. Head-to-Head Comparison of 8 Plasma Amyloid-β 42/40 Assays in Alzheimer Disease. JAMA Neurol. 2021 Nov

Subcellular Location

Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle.

UNIPROT

SwissProt: P05067 Human

SwissProt: P12023 Mouse

Synonyms

A4 antibody

A4_HUMAN antibody

AAA antibody

ABETA antibody

ABPP antibody

AICD-50 antibody

AICD-57 antibody

AICD-59 antibody

AID(50) antibody

AID(57) antibody

Expand

Images

  • Western blot analysis of beta Amyloid 1-40 on different peptides with Rabbit anti-beta Amyloid 1-40 antibody (<a href="/products/HA751034" style="font-weight: bold;text-decoration: underline;">HA751034</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human Aβ37 full length peptide (negative)<br />Lane 2: Human Aβ38 full length peptide (negative)<br />Lane 3: Human Aβ39 full length peptide (negative)<br />Lane 4: Human Aβ40 full length peptide (positive)<br />Lane 5: Human Aβ41 full length peptide (negative)<br />Lane 6: Human Aβ42 full length peptide (negative)<br />Lane 7: Human Aβ43 full length peptide (negative)<br />Lane 8: Mouse Aβ40 full length peptide (positive)<br /><br />Lysates/proteins at 100 ng/Lane.<br /><br />Predicted band size: 4 kDa<br />Observed band size: 4 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751034" style="font-weight: bold;text-decoration: underline;">HA751034</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of beta Amyloid 1-40 on different peptides with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/1,000 dilution.

    Lane 1: Human Aβ37 full length peptide (negative)
    Lane 2: Human Aβ38 full length peptide (negative)
    Lane 3: Human Aβ39 full length peptide (negative)
    Lane 4: Human Aβ40 full length peptide (positive)
    Lane 5: Human Aβ41 full length peptide (negative)
    Lane 6: Human Aβ42 full length peptide (negative)
    Lane 7: Human Aβ43 full length peptide (negative)
    Lane 8: Mouse Aβ40 full length peptide (positive)

    Lysates/proteins at 100 ng/Lane.

    Predicted band size: 4 kDa
    Observed band size: 4 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751034) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-40 antibody (<a href="/products/HA751034" style="font-weight: bold;text-decoration: underline;">HA751034</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751034" style="font-weight: bold;text-decoration: underline;">HA751034</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded normal mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-40 antibody (<a href="/products/HA751034" style="font-weight: bold;text-decoration: underline;">HA751034</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751034" style="font-weight: bold;text-decoration: underline;">HA751034</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded normal mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue labeling beta Amyloid 1-40 with Rabbit anti-beta Amyloid 1-40 antibody (<a href="/products/HA751034" style="font-weight: bold;text-decoration: underline;">HA751034</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA751034" style="font-weight: bold;text-decoration: underline;">HA751034</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue labeling beta Amyloid 1-40 with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunofluorescence analysis of paraffin-embedded normal mouse brain tissue (negative control) labeling beta Amyloid 1-40 with Rabbit anti-beta Amyloid 1-40 antibody (<a href="/products/HA751034" style="font-weight: bold;text-decoration: underline;">HA751034</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA751034" style="font-weight: bold;text-decoration: underline;">HA751034</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    ☑ Relative expression (RE)

    Immunofluorescence analysis of paraffin-embedded normal mouse brain tissue (negative control) labeling beta Amyloid 1-40 with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751034, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

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