CD40 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH05-90] (HA751037)
Catalog# HA751037
Rabbit Monoclonal to CD40
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
-
Cynomolgus monkey
-
Pig
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD40 on different lysates with Rabbit anti-CD40 antibody (HA751037) at 1/1,000 dilution.
Lane 1: Raji cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: U-2 OS cell lysate
Lane 4: HEK-293 cell lysate (negative)
Lane 5: Ramos cell lysate
Lane 6: A549 cell lysate (negative)
Lane 7: Daudi cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 40 kDa
Exposure time: Lane 1-2: 1 minute; Lane 3-7: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751037) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD40 on different lysates with Rabbit anti-CD40 antibody (HA751037) at 1/1,000 dilution.
Lane 1: Raji cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: U-2 OS cell lysate
Lane 4: HEK-293 cell lysate (negative)
Lane 5: Ramos cell lysate
Lane 6: A549 cell lysate (negative)
Lane 7: Daudi cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 40 kDa
Exposure time: Lane 1-2: 1 minute; Lane 3-7: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751037) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.