MAP3K1 Recombinant Rabbit Monoclonal Antibody [PSH06-10] - BSA and Azide free
Catalog# HA751048
MAP3K1 Recombinant Rabbit Monoclonal Antibody [PSH06-10] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MAP3K1 Recombinant Rabbit Monoclonal Antibody [PSH06-10] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human MAP3K1 aa 1413-1512.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 165 kDa
Positive Control
A431 cell lysate, Jurkat cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, THP-1 cell lysate, HeLa cell lysate, HL-60 cell lysate, PC-12 cell lysate, Mouse spleen tissue lysate.
Conjugation
unconjugated
Clone Number
PSH06-10
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females.
Background References
1. Wang J et al. Genetic Control of MAP3K1 in Eye Development and Sex Differentiation. Cells. 2021 Dec
2. Aziz MA et al. MAP3K1 rs889312 polymorphism and cancer prognosis: A systematic review and meta-analysis. Cancer Rep (Hoboken). 2023 Jan
Subcellular Location
Cytoplasm, cytosol.
Synonyms
M3K1_HUMAN antibody
MAP3K1 antibody
MAPK/ERK kinase kinase 1 antibody
MAPKKK1 antibody
MEK kinase 1 antibody
MEKK 1 antibody
Mekk antibody
MEKK1 antibody
Mitogen activated protein kinase kinase kinase 1 antibody
Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody
ExpandImages
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This data was developed using HA722507, the same antibody clone in a different buffer formulation.
Western blot analysis of MAP3K1 on different lysates with Rabbit anti-MAP3K1 antibody (HA722507) at 1/2,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: THP-1 cell lysate (20 µg/Lane)
Lane 6: HeLa cell lysate (20 µg/Lane)
Lane 7: HL-60 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Mouse spleen tissue lysate (40 µg/Lane)
Predicted band size: 165 kDa
Observed band size: 130 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722507) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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