MAP3K1 Recombinant Rabbit Monoclonal Antibody [PSH06-10]
Overview
Product Name
MAP3K1 Recombinant Rabbit Monoclonal Antibody [PSH06-10]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human MAP3K1 aa 1413-1512.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 165 kDa
Positive Control
A431 cell lysate, Jurkat cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, THP-1 cell lysate, HeLa cell lysate, HL-60 cell lysate, PC-12 cell lysate, Mouse spleen tissue lysate.
Conjugation
unconjugated
Clone Number
PSH06-10
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
Target
Function
The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females.
Background References
1. Wang J et al. Genetic Control of MAP3K1 in Eye Development and Sex Differentiation. Cells. 2021 Dec
2. Aziz MA et al. MAP3K1 rs889312 polymorphism and cancer prognosis: A systematic review and meta-analysis. Cancer Rep (Hoboken). 2023 Jan
Subcellular Location
Cytoplasm, cytosol.
Synonyms
M3K1_HUMAN antibody
MAP3K1 antibody
MAPK/ERK kinase kinase 1 antibody
MAPKKK1 antibody
MEK kinase 1 antibody
MEKK 1 antibody
Mekk antibody
MEKK1 antibody
Mitogen activated protein kinase kinase kinase 1 antibody
Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody
ExpandImages
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Western blot analysis of MAP3K1 on different lysates with Rabbit anti-MAP3K1 antibody (HA722507) at 1/2,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: THP-1 cell lysate (20 µg/Lane)
Lane 6: HeLa cell lysate (20 µg/Lane)
Lane 7: HL-60 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Mouse spleen tissue lysate (40 µg/Lane)
Predicted band size: 165 kDa
Observed band size: 130 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722507) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Mechanism of Dendrobium officinale polysaccharide in alleviating Alzheimer's disease: Insights from metabolomics, lipidomics, and proteomics analysis
Journal: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2025.145531
IF: 8.5
Application: WB
Reactivity: Mouse
Publish date: 2025 Jun
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