LSD2 / AOF1 Recombinant Rabbit Monoclonal Antibody [PSH06-18] - BSA and Azide free
Catalog# HA751056
LSD2 / AOF1 Recombinant Rabbit Monoclonal Antibody [PSH06-18] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
LSD2 / AOF1 Recombinant Rabbit Monoclonal Antibody [PSH06-18] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human LSD2 aa 1-450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 92 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, HCT 116 cell lysate, K-562 cell lysate, A549 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, C6 cell lysate.
Conjugation
unconjugated
Clone Number
PSH06-18
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of GLYR1 to achieve such activity, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes.
Background References
1. Marabelli C., Marrocco B., Pilotto S., Chittori S., Picaud S., Marchese S., Ciossani G., Forneris F., Filippakopoulos P., Mattevi A. A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex. Cell Rep. 27:387-399.e7 (2019)
2. Chen F., Yang H., Dong Z., Fang J., Wang P., Zhu T., Gong W., Fang R., Shi Y.G., Xu Y. Structural insight into substrate recognition by histone demethylase LSD2/KDM1b. Cell Res. 23:306-309 (2013)
Subcellular Location
Chromosome. Nucleus.
Synonyms
amine oxidase (flavin containing) domain 1 antibody
amine oxidase, flavin containing 1 antibody
AOF1 antibody
bA204B7.3 antibody
C6orf193 antibody
dJ298J15.2 antibody
Flavin-containing amine oxidase domain-containing protein 1 antibody
FLJ33898 antibody
FLJ34109 antibody
FLJ43328 antibody
ExpandImages
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This data was developed using HA722515, the same antibody clone in a different buffer formulation.
Western blot analysis of LSD2 / AOF1 on different lysates with Rabbit anti-LSD2 / AOF1 antibody (HA722515) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HCT 116 cell lysate
Lane 4: K-562 cell lysate
Lane 5: A549 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: RAW264.7 cell lysate
Lane 9: Neuro-2a cell lysate
Lane 10: PC-12 cell lysate
Lane 11: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722515) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"