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Aquaporin 4 Recombinant Rabbit Monoclonal Antibody [PSH06-38] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751077

Aquaporin 4 Recombinant Rabbit Monoclonal Antibody [PSH06-38] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Tissue

  • IHC-Fr

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

Aquaporin 4 Recombinant Rabbit Monoclonal Antibody [PSH06-38] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human 224-323.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IF-Tissue, IHC-Fr, IP

Molecular Weight

Predicted band size: 35 kDa

Positive Control

Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, rat brain tissue, human kidney tissue, human brain tissue.

Conjugation

unconjugated

Clone Number

PSH06-38

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:2,000-1:10,000

  • IF-Tissue

  • 1:1,000

  • IHC-Fr

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Aquaporin-4, also known as AQP-4, is a water channel protein encoded by the AQP4 gene in humans. AQP-4 belongs to the aquaporin family of integral membrane proteins that conduct water through the cell membrane. A limited number of aquaporins are found within the central nervous system (CNS): AQP1, 3, 4, 5, 8, 9, and 11, but more exclusive representation of AQP1, 4, and 9 are found in the brain and spinal cord. AQP4 shows the largest presence in the cerebellum and spinal cord grey matter. In the CNS, AQP4 is the most prevalent aquaporin channel, specifically located at the perimicrovessel astrocyte foot processes, glia limitans, and ependyma. In addition, this channel is commonly found facilitating water movement near cerebrospinal fluid and vasculature. Aquaporin-4 was first identified in 1986. It was the first evidence of the existence of water transport channels. The method that was used to discover the existence of the transport channels was through knockout experiments. With this technique they were able to show the significant role of AQP4 in CNS injuries and brain water imbalances. In 1994 the channel was successfully cloned and initially named Mercury-Insensitive Water Channel.

Background References

1. Pittock SJ et al. Ravulizumab in Aquaporin-4-Positive Neuromyelitis Optica Spectrum Disorder. Ann Neurol. 2023 Jun

2. Kitchen P et al. Targeting Aquaporin-4 Subcellular Localization to Treat Central Nervous System Edema. Cell. 2020 May

Subcellular Location

Cell membrane, Basolateral cell membrane, Endosome membrane, Cell membrane, sarcolemma, Cell projection.

UNIPROT

SwissProt: P55087 Human

SwissProt: P55088 Mouse

SwissProt: P47863 Rat

Synonyms

AQP 4 antibody

AQP-4 antibody

AQP4 antibody

AQP4_HUMAN antibody

Aquaporin type 4 antibody

Aquaporin-4 antibody

Aquaporin4 antibody

HMIWC 2 antibody

HMIWC2 antibody

Mercurial insensitive water channel antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:1,000<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1:1,000

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:1,000<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1:1,000

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aquaporin 4 antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aquaporin 4 antibody (HA751077) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751077) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Aquaporin 4 antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Aquaporin 4 antibody (HA751077) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751077) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Aquaporin 4 antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Aquaporin 4 antibody (HA751077) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751077) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Aquaporin 4 antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Aquaporin 4 antibody (HA751077) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751077) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Aquaporin 4 antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Aquaporin 4 antibody (HA751077) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751077) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Aquaporin 4 on different lysates with Rabbit anti-Aquaporin 4 antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (no heat) (40 µg/Lane)<br />Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)<br />Lane 3: Rat brain tissue lysate (no heat) (40 µg/Lane)<br />Lane 4: Rat liver tissue lysate (negative) (40 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 16 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Aquaporin 4 on different lysates with Rabbit anti-Aquaporin 4 antibody (HA751077) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate (no heat) (40 µg/Lane)
    Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)
    Lane 3: Rat brain tissue lysate (no heat) (40 µg/Lane)
    Lane 4: Rat liver tissue lysate (negative) (40 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Predicted band size: 35 kDa
    Observed band size: 30 kDa

    Exposure time: 16 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751077) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1:1,000

    Application: IF-tissue

    Species: Mouse

    Site: Cerebral cortex

    Sample: Paraffin-embedded section

    Antibody concentration: 1:1,000

  • Aquaporin 4 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with <a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a> at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (<a href="/products/M1003-7" style="font-weight: bold;text-decoration: underline;">M1003-7</a>) at 1/100,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Mouse brain tissue lysate (input)<br />Lane 2: <a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a> IP in mouse brain tissue lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751077" style="font-weight: bold;text-decoration: underline;">HA751077</a> in mouse brain tissue lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 1 minute; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Aquaporin 4 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA751077 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751077 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/100,000 dilution was used for 1 hour at room temperature.

    Lane 1: Mouse brain tissue lysate (input)
    Lane 2: HA751077 IP in mouse brain tissue lysate
    Lane 3: Rabbit IgG instead of HA751077 in mouse brain tissue lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 1 minute; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"