Aquaporin 4 Recombinant Rabbit Monoclonal Antibody [PSH06-38]
Catalog# HA722672
Aquaporin 4 Recombinant Rabbit Monoclonal Antibody [PSH06-38]
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WB
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IHC-P
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IF-Tissue
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IHC-Fr
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IP
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Human
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Mouse
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Rat
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HA751077
不含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Aquaporin 4 Recombinant Rabbit Monoclonal Antibody [PSH06-38]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human 224-323.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IF-Tissue, IHC-Fr, IP
Molecular Weight
Predicted band size: 35 kDa
Positive Control
Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, rat brain tissue, human kidney tissue, human brain tissue.
Conjugation
unconjugated
Clone Number
PSH06-38
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:2,000-1:10,000
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IF-Tissue
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1:1,000
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IHC-Fr
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1:1,000
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IP
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1-2μg/sample
Target
Function
Aquaporin-4, also known as AQP-4, is a water channel protein encoded by the AQP4 gene in humans. AQP-4 belongs to the aquaporin family of integral membrane proteins that conduct water through the cell membrane. A limited number of aquaporins are found within the central nervous system (CNS): AQP1, 3, 4, 5, 8, 9, and 11, but more exclusive representation of AQP1, 4, and 9 are found in the brain and spinal cord. AQP4 shows the largest presence in the cerebellum and spinal cord grey matter. In the CNS, AQP4 is the most prevalent aquaporin channel, specifically located at the perimicrovessel astrocyte foot processes, glia limitans, and ependyma. In addition, this channel is commonly found facilitating water movement near cerebrospinal fluid and vasculature. Aquaporin-4 was first identified in 1986. It was the first evidence of the existence of water transport channels. The method that was used to discover the existence of the transport channels was through knockout experiments. With this technique they were able to show the significant role of AQP4 in CNS injuries and brain water imbalances. In 1994 the channel was successfully cloned and initially named Mercury-Insensitive Water Channel.
Background References
1. Pittock SJ et al. Ravulizumab in Aquaporin-4-Positive Neuromyelitis Optica Spectrum Disorder. Ann Neurol. 2023 Jun
2. Kitchen P et al. Targeting Aquaporin-4 Subcellular Localization to Treat Central Nervous System Edema. Cell. 2020 May
Subcellular Location
Cell membrane, Basolateral cell membrane, Endosome membrane, Cell membrane, sarcolemma, Cell projection.
Synonyms
AQP 4 antibody
AQP-4 antibody
AQP4 antibody
AQP4_HUMAN antibody
Aquaporin type 4 antibody
Aquaporin-4 antibody
Aquaporin4 antibody
HMIWC 2 antibody
HMIWC2 antibody
Mercurial insensitive water channel antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aquaporin 4 antibody (HA722672) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722672) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Aquaporin 4 antibody (HA722672) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722672) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Aquaporin 4 antibody (HA722672) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722672) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Aquaporin 4 antibody (HA722672) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722672) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Aquaporin 4 antibody (HA722672) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722672) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of Aquaporin 4 on different lysates with Rabbit anti-Aquaporin 4 antibody (HA722672) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat) (40 µg/Lane)
Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 3: Rat brain tissue lysate (no heat) (40 µg/Lane)
Lane 4: Rat liver tissue lysate (negative) (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 35 kDa
Observed band size: 30 kDa
Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722672) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IF-tissue
Species: Mouse
Site: Cerebral cortex
Sample: Paraffin-embedded section
Antibody concentration: 1:1,000 -
Aquaporin 4 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722672 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722672 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/100,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (input)
Lane 2: HA722672 IP in mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA722672 in mouse brain tissue lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"