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CSF-1-R Recombinant Rabbit Monoclonal Antibody [PSH06-70] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751099

CSF-1-R Recombinant Rabbit Monoclonal Antibody [PSH06-70] - BSA and Azide free

Application

  • WB

  • IF-Cell

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CSF-1-R Recombinant Rabbit Monoclonal Antibody [PSH06-70] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse CSF-1-R aa 1-536.

Species Reactivity

Mouse

Validated Applications

WB, IF-Cell

Target Molecular Weight

Predicted band size: 109 kDa

Positive Control

RAW264.7 cell lysate, J774A.1 cell lysate, RAW264.7.

Conjugation

unconjugated

Clone Number

PSH06-70

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), and CD115 (Cluster of Differentiation 115), is a cell-surface protein encoded by the human CSF1R gene (known also as c-FMS). CSF1R is a receptor that can be activated by two ligands: colony stimulating factor 1 (CSF-1) and interleukin-34 (IL-34). CSF1R is highly expressed in myeloid cells, and CSF1R signaling is necessary for the survival, proliferation, and differentiation of many myeloid cell types in vivo and in vitro. CSF1R signaling is involved in many diseases and is targeted in therapies for cancer, neurodegeneration, and inflammatory bone diseases.

Background References

1. Wen J et al. CSF1R inhibitors are emerging immunotherapeutic drugs for cancer treatment. Eur J Med Chem. 2023 Jan

2. Hume DA et al. CSF1R as a Therapeutic Target in Bone Diseases: Obvious but Not so Simple. Curr Osteoporos Rep. 2022 Dec

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P09581 Mouse

Synonyms

C FMS antibody

CD 115 antibody

CD115 antibody

CD115 antigen antibody

CFMS antibody

Colony stimulating factor 1 receptor antibody

Colony stimulating factor I receptor antibody

CSF 1 R antibody

CSF 1R antibody

CSF-1 receptor antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA722742" style="font-weight: bold;text-decoration: underline;">HA722742</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of CSF-1-R on different lysates with Rabbit anti-CSF-1-R antibody (<a href="/products/HA722742" style="font-weight: bold;text-decoration: underline;">HA722742</a>) at 1/2,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate<br />Lane 2: J774A.1 cell lysate<br />Lane 3: Neuro-2a cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 109 kDa<br />Observed band size: 90-160 kDa<br /><br />Exposure time: 40 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722742" style="font-weight: bold;text-decoration: underline;">HA722742</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    This data was developed using HA722742, the same antibody clone in a different buffer formulation.
    Western blot analysis of CSF-1-R on different lysates with Rabbit anti-CSF-1-R antibody (HA722742) at 1/2,000 dilution.

    Lane 1: RAW264.7 cell lysate
    Lane 2: J774A.1 cell lysate
    Lane 3: Neuro-2a cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 109 kDa
    Observed band size: 90-160 kDa

    Exposure time: 40 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722742) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA722742" style="font-weight: bold;text-decoration: underline;">HA722742</a>, the same antibody clone in a different buffer formulation.<br />Immunocytochemistry analysis of RAW264.7 (positive) and Neuro-2a (negative) labeling CSF-1-R with Rabbit anti-CSF-1-R antibody (<a href="/products/HA722742" style="font-weight: bold;text-decoration: underline;">HA722742</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSF-1-R antibody (<a href="/products/HA722742" style="font-weight: bold;text-decoration: underline;">HA722742</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Relative expression (RE)

    This data was developed using HA722742, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of RAW264.7 (positive) and Neuro-2a (negative) labeling CSF-1-R with Rabbit anti-CSF-1-R antibody (HA722742) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSF-1-R antibody (HA722742) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"