CSF-1-R Recombinant Rabbit Monoclonal Antibody [PSH06-70]
Catalog# HA722742
CSF-1-R Recombinant Rabbit Monoclonal Antibody [PSH06-70]
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WB
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IF-Cell
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Mouse
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unconjugated
Overview
Product Name
CSF-1-R Recombinant Rabbit Monoclonal Antibody [PSH06-70]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse CSF-1-R aa 1-536.
Species Reactivity
Mouse
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 109 kDa
Positive Control
RAW264.7 cell lysate, J774A.1 cell lysate, RAW264.7.
Conjugation
unconjugated
Clone Number
PSH06-70
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:100
Target
Function
Colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), and CD115 (Cluster of Differentiation 115), is a cell-surface protein encoded by the human CSF1R gene (known also as c-FMS). CSF1R is a receptor that can be activated by two ligands: colony stimulating factor 1 (CSF-1) and interleukin-34 (IL-34). CSF1R is highly expressed in myeloid cells, and CSF1R signaling is necessary for the survival, proliferation, and differentiation of many myeloid cell types in vivo and in vitro. CSF1R signaling is involved in many diseases and is targeted in therapies for cancer, neurodegeneration, and inflammatory bone diseases.
Background References
1. Wen J et al. CSF1R inhibitors are emerging immunotherapeutic drugs for cancer treatment. Eur J Med Chem. 2023 Jan
2. Hume DA et al. CSF1R as a Therapeutic Target in Bone Diseases: Obvious but Not so Simple. Curr Osteoporos Rep. 2022 Dec
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
C FMS antibody
CD 115 antibody
CD115 antibody
CD115 antigen antibody
CFMS antibody
Colony stimulating factor 1 receptor antibody
Colony stimulating factor I receptor antibody
CSF 1 R antibody
CSF 1R antibody
CSF-1 receptor antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CSF-1-R on different lysates with Rabbit anti-CSF-1-R antibody (HA722742) at 1/2,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: J774A.1 cell lysate
Lane 3: Neuro-2a cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 109 kDa
Observed band size: 90-160 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722742) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of RAW264.7 (positive) and Neuro-2a (negative) labeling CSF-1-R with Rabbit anti-CSF-1-R antibody (HA722742) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSF-1-R antibody (HA722742) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"