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CSN7b Recombinant Rabbit Monoclonal Antibody [PSH06-84] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751107

CSN7b Recombinant Rabbit Monoclonal Antibody [PSH06-84] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CSN7b Recombinant Rabbit Monoclonal Antibody [PSH06-84] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human CSN7b aa 215-264.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 30 kDa

Positive Control

293T cell lysate, HeLa cell lysate, Jurkat cell lysate, HL-60 cell lysate, HT-29 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.

Conjugation

unconjugated

Clone Number

PSH06-84

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in protein deneddylation. Located in nucleoplasm. Part of COP9 signalosome.

Background References

1. Wang J et al. CSN7B defines a variant COP9 signalosome complex with distinct function in DNA damage response. Cell Rep. 2021 Jan

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q9H9Q2 Human

SwissProt: Q8BV13 Mouse

Entrez Gene: 363273 Rat

Synonyms

COP9 Complex Homolog Subunit 7b antibody

COP9 Constitutive Photomorphogenic Homolog Subunit 7b antibody

COP9 Signalasome Subunit 7b antibody

COP9 signalosome complex subunit 7b antibody

COPS7B antibody

CSN7B_HUMAN antibody

JAB1 Containing Signalosome Subunit 7b antibody

JAB1-containing signalosome subunit 7b antibody

SGN7b antibody

Signalosome subunit 7b antibody

Images

  • This data was developed using <a href="/products/HA722758" style="font-weight: bold;text-decoration: underline;">HA722758</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of CSN7b on different lysates with Rabbit anti-CSN7b antibody (<a href="/products/HA722758" style="font-weight: bold;text-decoration: underline;">HA722758</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: HL-60 cell lysate<br />Lane 5: HT-29 cell lysate<br />Lane 6: RAW264.7 cell lysate<br />Lane 7: NIH/3T3 cell lysate<br />Lane 8: C6 cell lysate<br />Lane 9: PC-12 cell lysate<br />Lane 10: Mouse testis tissue lysate<br />Lane 11: Rat testis tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 30 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722758" style="font-weight: bold;text-decoration: underline;">HA722758</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA722758, the same antibody clone in a different buffer formulation.
    Western blot analysis of CSN7b on different lysates with Rabbit anti-CSN7b antibody (HA722758) at 1/1,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: HL-60 cell lysate
    Lane 5: HT-29 cell lysate
    Lane 6: RAW264.7 cell lysate
    Lane 7: NIH/3T3 cell lysate
    Lane 8: C6 cell lysate
    Lane 9: PC-12 cell lysate
    Lane 10: Mouse testis tissue lysate
    Lane 11: Rat testis tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722758) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"