CSN7b Recombinant Rabbit Monoclonal Antibody [PSH06-84]
Catalog# HA722758
CSN7b Recombinant Rabbit Monoclonal Antibody [PSH06-84]
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WB
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Human
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Mouse
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Rat
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HA751107
不含抗保成分
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unconjugated
Overview
Product Name
CSN7b Recombinant Rabbit Monoclonal Antibody [PSH06-84]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CSN7b aa 215-264.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 30 kDa
Positive Control
293T cell lysate, HeLa cell lysate, Jurkat cell lysate, HL-60 cell lysate, HT-29 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
PSH06-84
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in protein deneddylation. Located in nucleoplasm. Part of COP9 signalosome.
Background References
1. Wang J et al. CSN7B defines a variant COP9 signalosome complex with distinct function in DNA damage response. Cell Rep. 2021 Jan
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
COP9 Complex Homolog Subunit 7b antibody
COP9 Constitutive Photomorphogenic Homolog Subunit 7b antibody
COP9 Signalasome Subunit 7b antibody
COP9 signalosome complex subunit 7b antibody
COPS7B antibody
CSN7B_HUMAN antibody
JAB1 Containing Signalosome Subunit 7b antibody
JAB1-containing signalosome subunit 7b antibody
SGN7b antibody
Signalosome subunit 7b antibody
Images
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Western blot analysis of CSN7b on different lysates with Rabbit anti-CSN7b antibody (HA722758) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HL-60 cell lysate
Lane 5: HT-29 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: C6 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: Mouse testis tissue lysate
Lane 11: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722758) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"