Phospho-STAT6 (Y641) Recombinant Rabbit Monoclonal Antibody [PSH06-98] - BSA and Azide free
Catalog# HA751125
Phospho-STAT6 (Y641) Recombinant Rabbit Monoclonal Antibody [PSH06-98] - BSA and Azide free
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WB
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Human
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Mouse
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HA722776
含抗保成分
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unconjugated
Overview
Product Name
Phospho-STAT6 (Y641) Recombinant Rabbit Monoclonal Antibody [PSH06-98] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Tyr641 of human p53 aa 611-660..
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 94 kDa
Positive Control
Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate, J774A.1 treated with 10ng/mL mIL-4 for 2 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH06-98
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
Target
Function
Signal transducer and activator of transcription 6 (STAT6) is a transcription factor that belongs to the Signal Transducer and Activator of Transcription (STAT) family of proteins. The proteins of STAT family transmit signals from a receptor complex to the nucleus and activate gene expression. Similarly as other STAT family proteins, STAT6 is also activated by growth factors and cytokines. STAT6 is mainly activated by cytokines interleukin-4 and interleukin-13.
Background References
1. Yang Y et al. STAT6 inhibits ferroptosis and alleviates acute lung injury via regulating P53/SLC7A11 pathway. Cell Death Dis. 2022 Jun
2. Takeuchi I et al. STAT6 gain-of-function variant exacerbates multiple allergic symptoms. J Allergy Clin Immunol. 2023 May
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
12S1644 antibody
D12S1644 antibody
IL 4 STAT antibody
IL-4 Stat antibody
IL4 STAT antibody
Interleukin 4 Induced antibody
Interleukin 4 Induced Transcription Factor IL4 STAT antibody
Signal transducer and activator of transcription 6 antibody
Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA751125) at 1/1,000 dilution.
Lane 1: Daudi cell lysate
Lane 2: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate
Lane 3: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 94 kDa
Observed band size: 100 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751125) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA751125) at 1/2,000 dilution.
Lane 1: J774A.1 cell lysate
Lane 2: J774A.1 treated with 10ng/mL mIL-4 for 2 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 94 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751125) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"