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Phospho-STAT6 (Y641) Recombinant Rabbit Monoclonal Antibody [PSH06-98]

Usd: 385

Specification

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Catalog# HA722776

Phospho-STAT6 (Y641) Recombinant Rabbit Monoclonal Antibody [PSH06-98]

Application

  • WB

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-STAT6 (Y641) Recombinant Rabbit Monoclonal Antibody [PSH06-98]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Tyr641 of human p53 aa 611-660..

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 94 kDa

Positive Control

Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate, J774A.1 treated with 10ng/mL mIL-4 for 2 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH06-98

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

Signal transducer and activator of transcription 6 (STAT6) is a transcription factor that belongs to the Signal Transducer and Activator of Transcription (STAT) family of proteins. The proteins of STAT family transmit signals from a receptor complex to the nucleus and activate gene expression. Similarly as other STAT family proteins, STAT6 is also activated by growth factors and cytokines. STAT6 is mainly activated by cytokines interleukin-4 and interleukin-13.

Background References

1. Yang Y et al. STAT6 inhibits ferroptosis and alleviates acute lung injury via regulating P53/SLC7A11 pathway. Cell Death Dis. 2022 Jun

2. Takeuchi I et al. STAT6 gain-of-function variant exacerbates multiple allergic symptoms. J Allergy Clin Immunol. 2023 May

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P42226 Human

SwissProt: P52633 Mouse

Synonyms

12S1644 antibody

D12S1644 antibody

IL 4 STAT antibody

IL-4 Stat antibody

IL4 STAT antibody

Interleukin 4 Induced antibody

Interleukin 4 Induced Transcription Factor IL4 STAT antibody

Signal transducer and activator of transcription 6 antibody

Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody

Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (<a href="/products/HA722776" style="font-weight: bold;text-decoration: underline;">HA722776</a>) at 1/1,000 dilution.<br /><br />Lane 1: Daudi cell lysate<br />Lane 2: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate<br />Lane 3: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 94 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722776" style="font-weight: bold;text-decoration: underline;">HA722776</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA722776) at 1/1,000 dilution.

    Lane 1: Daudi cell lysate
    Lane 2: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate
    Lane 3: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 94 kDa
    Observed band size: 100 kDa

    Exposure time: 30 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (<a href="/products/HA722776" style="font-weight: bold;text-decoration: underline;">HA722776</a>) at 1/2,000 dilution.<br /><br />Lane 1: J774A.1 cell lysate<br />Lane 2: J774A.1 treated with 10ng/mL mIL-4 for 2 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 94 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722776" style="font-weight: bold;text-decoration: underline;">HA722776</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA722776) at 1/2,000 dilution.

    Lane 1: J774A.1 cell lysate
    Lane 2: J774A.1 treated with 10ng/mL mIL-4 for 2 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 94 kDa
    Observed band size: 100 kDa

    Exposure time: 2 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722776) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation