ATF4 Recombinant Rabbit Monoclonal Antibody [PSH07-08] - BSA and Azide free
Catalog# HA751130
ATF4 Recombinant Rabbit Monoclonal Antibody [PSH07-08] - BSA and Azide free
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WB
-
IP
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Human
-
Mouse
-
Rat
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unconjugated
Overview
Product Name
ATF4 Recombinant Rabbit Monoclonal Antibody [PSH07-08] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ATF-4 aa 1-300.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 39 kDa
Positive Control
HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate, C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate, HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate, HeLa treated with 250μM CoCl2 for 6 hours cell lysate, NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH07-08
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
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IP
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1-2μg/sample
Target
Function
Activating transcription factor 4 (tax-responsive enhancer element B67), also known as ATF4, is a protein that in humans is encoded by the ATF4 gene. ATF4 transcription factor is also known to play role in osteoblast differentiation along with RUNX2 and osterix. Terminal osteoblast differentiation, represented by matrix mineralization, is significantly inhibited by the inactivation of JNK. JNK inactivation downregulates expression of ATF-4 and, subsequently, matrix mineralization. IMPACT protein regulates ATF4 in C. elegans to promote lifespan. ATF4 is also involved in the cannabinoid Δ9-tetrahydrocannabinol–induced apoptosis in cancer cells, by the proapoptotic role of the stress protein p8 via its upregulation of the endoplasmic reticulum stress-related genes ATF4, CHOP, and TRB3.
Background References
1. He F et al. ATF4 suppresses hepatocarcinogenesis by inducing SLC7A11 (xCT) to block stress-related ferroptosis. J Hepatol. 2023 Aug
2. Xiao Y et al. Advances in the roles of ATF4 in osteoporosis. Biomed Pharmacother. 2023 Dec
Subcellular Location
Nucleus, Nucleus speckle, Cytoplasm, Cell membrane, cytoskeleton, microtubule organizing center, centrosome.
Synonyms
Activating transcription factor 4 antibody
ATF 4 antibody
ATF4 antibody
ATF4 protein antibody
ATF4_HUMAN antibody
cAMP-dependent transcription factor ATF-4 antibody
cAMP-responsive element-binding protein 2 antibody
CREB 2 antibody
CREB-2 antibody
CREB2 antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of ATF4 on different lysates with Rabbit anti-ATF4 antibody (HA751130) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)
Predicted band size: 39 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751130) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of ATF4 on different lysates with Rabbit anti-ATF4 antibody (HA751130) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: HeLa treated with 250μM CoCl2 for 6 hours cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)
Predicted band size: 39 kDa
Observed band size: 50 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751130) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
ATF4 was immunoprecipitated from 0.2 mg HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate with HA751130 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751130 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (input)
Lane 2: HA751130 IP in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate
Lane 3: Rabbit IgG instead of HA751130 in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 40 seconds; ECL: K1802
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
ATF4 Recombinant Rabbit Monoclonal Antibody [SD20-92]
Application: WB,IF-Cell,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
ATF4 Recombinant Rabbit Monoclonal Antibody [PSH07-08]
Application: WB,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
