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ATF4 Recombinant Rabbit Monoclonal Antibody [PSH07-08]

Usd: 385

Specification

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Catalog# HA722796

ATF4 Recombinant Rabbit Monoclonal Antibody [PSH07-08]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

ATF4 Recombinant Rabbit Monoclonal Antibody [PSH07-08]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ATF-4 aa 1-300.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 39 kDa

Positive Control

HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate, C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate, HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate, HeLa treated with 250μM CoCl2 for 6 hours cell lysate, NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH07-08

Reactivity Data

WB IP
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Activating transcription factor 4 (tax-responsive enhancer element B67), also known as ATF4, is a protein that in humans is encoded by the ATF4 gene. ATF4 transcription factor is also known to play role in osteoblast differentiation along with RUNX2 and osterix. Terminal osteoblast differentiation, represented by matrix mineralization, is significantly inhibited by the inactivation of JNK. JNK inactivation downregulates expression of ATF-4 and, subsequently, matrix mineralization. IMPACT protein regulates ATF4 in C. elegans to promote lifespan. ATF4 is also involved in the cannabinoid Δ9-tetrahydrocannabinol–induced apoptosis in cancer cells, by the proapoptotic role of the stress protein p8 via its upregulation of the endoplasmic reticulum stress-related genes ATF4, CHOP, and TRB3.

Background References

1. He F et al. ATF4 suppresses hepatocarcinogenesis by inducing SLC7A11 (xCT) to block stress-related ferroptosis. J Hepatol. 2023 Aug

2. Xiao Y et al. Advances in the roles of ATF4 in osteoporosis. Biomed Pharmacother. 2023 Dec

Subcellular Location

Nucleus, Nucleus speckle, Cytoplasm, Cell membrane, cytoskeleton, microtubule organizing center, centrosome.

UNIPROT

SwissProt: P18848 Human

SwissProt: Q06507 Mouse

SwissProt: Q9ES19 Rat

Synonyms

Activating transcription factor 4 antibody

ATF 4 antibody

ATF4 antibody

ATF4 protein antibody

ATF4_HUMAN antibody

cAMP-dependent transcription factor ATF-4 antibody

cAMP-responsive element-binding protein 2 antibody

CREB 2 antibody

CREB-2 antibody

CREB2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of ATF4 on different lysates with Rabbit anti-ATF4 antibody (<a href="/products/HA722796" style="font-weight: bold;text-decoration: underline;">HA722796</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (20 µg/Lane)<br />Lane 2: HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)<br />Lane 3: C6 cell lysate (20 µg/Lane)<br />Lane 4: C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)<br /><br />Predicted band size: 39 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722796" style="font-weight: bold;text-decoration: underline;">HA722796</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of ATF4 on different lysates with Rabbit anti-ATF4 antibody (HA722796) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate (20 µg/Lane)
    Lane 2: HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)
    Lane 3: C6 cell lysate (20 µg/Lane)
    Lane 4: C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)

    Predicted band size: 39 kDa
    Observed band size: 50 kDa

    Exposure time: 2 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722796) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of ATF4 on different lysates with Rabbit anti-ATF4 antibody (<a href="/products/HA722796" style="font-weight: bold;text-decoration: underline;">HA722796</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)<br />Lane 3: HeLa cell lysate (20 µg/Lane)<br />Lane 4: HeLa treated with 250μM CoCl2 for 6 hours cell lysate (20 µg/Lane)<br />Lane 5: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 6: NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)<br /><br />Predicted band size: 39 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 59 seconds; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722796" style="font-weight: bold;text-decoration: underline;">HA722796</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of ATF4 on different lysates with Rabbit anti-ATF4 antibody (HA722796) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)
    Lane 3: HeLa cell lysate (20 µg/Lane)
    Lane 4: HeLa treated with 250μM CoCl2 for 6 hours cell lysate (20 µg/Lane)
    Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 6: NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane)

    Predicted band size: 39 kDa
    Observed band size: 50 kDa

    Exposure time: 59 seconds; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722796) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />ATF4 was immunoprecipitated from 0.2 mg HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate with <a href="/products/HA722796" style="font-weight: bold;text-decoration: underline;">HA722796</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722796" style="font-weight: bold;text-decoration: underline;">HA722796</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (input)<br />Lane 2: <a href="/products/HA722796" style="font-weight: bold;text-decoration: underline;">HA722796</a> IP in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722796" style="font-weight: bold;text-decoration: underline;">HA722796</a> in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 40 seconds; ECL: <a href="/products/K1802" style="font-weight: bold;text-decoration: underline;">K1802</a>

    ☑ Cell treatment (CT)

    ATF4 was immunoprecipitated from 0.2 mg HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate with HA722796 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722796 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (input)
    Lane 2: HA722796 IP in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate
    Lane 3: Rabbit IgG instead of HA722796 in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 40 seconds; ECL: K1802

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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