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Retinoic Acid Receptor gamma Recombinant Rabbit Monoclonal Antibody [PSH07-19] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751140

Retinoic Acid Receptor gamma Recombinant Rabbit Monoclonal Antibody [PSH07-19] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Retinoic Acid Receptor gamma Recombinant Rabbit Monoclonal Antibody [PSH07-19] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human RARG aa 155-454.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 50 kDa

Positive Control

SK-Br-3 cell lysate, T-47D cell lysate, A375 cell lysate, HeLa cell lysate, MEF cell lysate, Mouse skin tissue lysate, Rat skin tissue lysate, human skin tissue, mouse skin tissue, rat skin tissue.

Conjugation

unconjugated

Clone Number

PSH07-19

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:1,000-1:5,000

Target

Function

Retinoic acid receptor gamma (RAR-γ), also known as NR1B3 (nuclear receptor subfamily 1, group B, member 3) is a nuclear receptor encoded by the RARG gene. Adapalene selectively targets retinoic acid receptor beta and retinoic acid receptor gamma and its agonism of the gamma subtype is largely responsible for adapalene's observed effects.

Background References

1. Ji K et al. Retinoic acid receptor gamma is required for proliferation of pancreatic cancer cells. Cell Biol Int. 2023 Jan

2. Powała K et al. Molecular Interactions of Selective Agonists and Antagonists with the Retinoic Acid Receptor gamma. Int J Mol Sci. 2024 Jun

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: P13631 Human

SwissProt: P18911 Mouse

Entrez Gene: 685072 Rat

Synonyms

NR1B3 antibody

Nuclear receptor subfamily 1 group B member 3 antibody

RAR gamma antibody

RAR-gamma antibody

RARC antibody

Rarg antibody

RARG_HUMAN antibody

Retinoic acid receptor gamma 2 antibody

Retinoic acid receptor gamma antibody

Retinoic acid receptor, gamma antibody

Images

  • Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (<a href="/products/HA751140" style="font-weight: bold;text-decoration: underline;">HA751140</a>) at 1/2,000 dilution.<br /><br />Lane 1: SK-Br-3 cell lysate (20 µg/Lane)<br />Lane 2: T-47D cell lysate (20 µg/Lane)<br />Lane 3: A375 cell lysate (20 µg/Lane)<br />Lane 4: HeLa cell lysate (20 µg/Lane)<br />Lane 5: MEF cell lysate (20 µg/Lane)<br />Lane 6: Mouse skin tissue lysate (40 µg/Lane)<br />Lane 7: Rat skin tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 50 kDa<br />Observed band size: 58 kDa<br /><br />Exposure time: 1 minute 18 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751140" style="font-weight: bold;text-decoration: underline;">HA751140</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/2,000 dilution.

    Lane 1: SK-Br-3 cell lysate (20 µg/Lane)
    Lane 2: T-47D cell lysate (20 µg/Lane)
    Lane 3: A375 cell lysate (20 µg/Lane)
    Lane 4: HeLa cell lysate (20 µg/Lane)
    Lane 5: MEF cell lysate (20 µg/Lane)
    Lane 6: Mouse skin tissue lysate (40 µg/Lane)
    Lane 7: Rat skin tissue lysate (40 µg/Lane)

    Predicted band size: 50 kDa
    Observed band size: 58 kDa

    Exposure time: 1 minute 18 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751140) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (<a href="/products/HA751140" style="font-weight: bold;text-decoration: underline;">HA751140</a>) at 1/5,000 dilution.<br /><br />Lane 1: Human RARG recombinant protein, 20ng/Lane<br />Lane 2: Human RARA recombinant protein, 20ng/Lane<br />Lane 3: Human RARB recombinant protein, 20ng/Lane<br /><br />Exposure time: 8 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751140" style="font-weight: bold;text-decoration: underline;">HA751140</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/5,000 dilution.

    Lane 1: Human RARG recombinant protein, 20ng/Lane
    Lane 2: Human RARA recombinant protein, 20ng/Lane
    Lane 3: Human RARB recombinant protein, 20ng/Lane

    Exposure time: 8 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751140) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (<a href="/products/HA751140" style="font-weight: bold;text-decoration: underline;">HA751140</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751140" style="font-weight: bold;text-decoration: underline;">HA751140</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751140) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (<a href="/products/HA751140" style="font-weight: bold;text-decoration: underline;">HA751140</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751140" style="font-weight: bold;text-decoration: underline;">HA751140</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (<a href="/products/HA751140" style="font-weight: bold;text-decoration: underline;">HA751140</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751140" style="font-weight: bold;text-decoration: underline;">HA751140</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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