Hormone sensitive lipase / HSL Recombinant Rabbit Monoclonal Antibody [PSH07-22] - BSA and Azide free

This data was developed using HA722810, the same antibody clone in a different buffer formulation.
Western blot analysis of Hormone sensitive lipase / HSL on different lysates with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: SH-SY5Y cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 117 kDa
Observed band size: 90 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722810) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

☑ Relative expression (RE)

This data was developed using HA722810, the same antibody clone in a different buffer formulation.
Western blot analysis of Hormone sensitive lipase / HSL on different lysates with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/1,000 dilution.

Lane 1: Human liver tissue lysate (negative)
Lane 2: Mouse brown adipose tissue lysate
Lane 3: Rat brown adipose tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 117 kDa
Observed band size: 90 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722810) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

This data was developed using HA722810, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA722810, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA722810, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA722810, the same antibody clone in a different buffer formulation.
Hormone sensitive lipase / HSL was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722810 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722810 at 1/3,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: Jurkat cell lysate (input)
Lane 2: HA722810 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of HA722810 in Jurkat cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 46 seconds; ECL: K1801

This data was developed using HA722810, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)

Species: Human
Tissue: Breast
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× PBS
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA722810, 1/1,000, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.