Hormone sensitive lipase / HSL Recombinant Rabbit Monoclonal Antibody [PSH07-22]
Catalog# HA722810
Hormone sensitive lipase / HSL Recombinant Rabbit Monoclonal Antibody [PSH07-22]
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WB
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IHC-P
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IP
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mIHC
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IF-Tissue
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Human
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Mouse
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Rat
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HA751143
不含抗保成分
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unconjugated
Overview
Product Name
Hormone sensitive lipase / HSL Recombinant Rabbit Monoclonal Antibody [PSH07-22]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human HSL aa 931-980.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP, mIHC, IF-Tissue
Molecular Weight
Predicted band size: 117 kDa
Positive Control
Jurkat cell lysate, SH-SY5Y cell lysate, Mouse brown adipose tissue lysate, Rat brown adipose tissue lysate, human breast tissue, mouse breast tissue, rat breast tissue.
Conjugation
unconjugated
Clone Number
PSH07-22
Reactivity Data
| WB | IHC-P | IP | mIHC | IF-Tissue | |
|---|---|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:30,000
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IP
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1-2μg/sample
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mIHC
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1:30,000
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IF-Tissue
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1:1,000
Target
Function
The main function of hormone-sensitive lipase is to mobilize stored fats. HSL functions to hydrolyze either a fatty acid from a triacylglycerol molecule, freeing a fatty acid and diglyceride, or a fatty acid from a diacylglycerol molecule, freeing a fatty acid and monoglyceride. This process allows energy metabolism in mammals. Although HSL is able to catalyze hydrolysis of triglycerides and diglycerides, another enzyme found in adipose tissue, adipose triglyceride lipase (ATGL), has a higher affinity for triglycerides than HSL, and ATGL predominantly acts as the enzyme for triglyceride-specific hydrolysis in the adipocyte. Hormone-sensitive lipase, which has 11-fold greater affinity for diglycerides than triglycerides, predominantly cleaves these diglycerides, forming 2-monoglyceride and a free fatty acid.
Background References
1. Althaher AR. An Overview of Hormone-Sensitive Lipase (HSL). ScientificWorldJournal. 2022 Dec
2. Shen WJ et al. Hormone sensitive lipase ablation promotes bone regeneration. Biochim Biophys Acta Mol Basis Dis. 2022 Sep
Subcellular Location
Cell membrane, Membrane, caveola, Cytoplasm, cytosol, Lipid droplet.
Synonyms
Hormone sensitive lipase antibody
Hormone sensitive lipase testicular isoform antibody
Hormone-sensitive lipase antibody
HSL antibody
LHS antibody
Lipase hormone sensitive antibody
LIPE antibody
LIPS_HUMAN antibody
Images
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Western blot analysis of Hormone sensitive lipase / HSL on different lysates with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 117 kDa
Observed band size: 90 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722810) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Western blot analysis of Hormone sensitive lipase / HSL on different lysates with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/1,000 dilution.
Lane 1: Human liver tissue lysate (negative)
Lane 2: Mouse brown adipose tissue lysate
Lane 3: Rat brown adipose tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 117 kDa
Observed band size: 90 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722810) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Hormone sensitive lipase / HSL was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722810 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722810 at 1/3,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (input)
Lane 2: HA722810 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of HA722810 in Jurkat cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 46 seconds; ECL: K1801 -
Application: Immunofluorescence (IF-tissue)
Species: Human
Tissue: Breast
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× PBS
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA722810, 1/1,000, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Hormone sensitive lipase / HSL Recombinant Rabbit Monoclonal Antibody [PSH07-22] - BSA and Azide free
Application: WB,IHC-P,IP,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
