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ERK8 Recombinant Rabbit Monoclonal Antibody [PSH07-23] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751144

ERK8 Recombinant Rabbit Monoclonal Antibody [PSH07-23] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ERK8 Recombinant Rabbit Monoclonal Antibody [PSH07-23] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ERK8 aa 1-400.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 60 kDa

Positive Control

Raji cell lysate, Mouse kidney tissue lysate, Mouse lung tissue lysate, Rat kidney tissue lysate, Rat lung tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

PSH07-23

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Mitogen-activated protein kinase 15, also known as MAPK15, ERK7, or ERK8, is an enzyme that in humans is encoded by the MAPK15 gene. The protein encoded by this gene is a member of the MAP (mitogen-activated protein) kinase family. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), and are involved in signaling cascades that regulate a number of cellular processes, including proliferation, differentiation, and transcriptional regulation. In vertebrate models, ERK8 is not constitutively active, and exhibits relatively low basal kinase activity. It contains two SH3 (SRC homology 3) binding motifs in its C-terminal region, and is likely activated by an SRC-dependent signaling pathway. SRC is a non-receptor tyrosine kinase (and proto-oncogene) that has been implicated in cancer growth and progression in humans when it is overexpressed. The exact function of MAPK15 is unknown, though a number of studies have implicated the enzyme in various cellular pathways.

Background References

1. Franci L et al. MAPK15 protects from oxidative stress-dependent cellular senescence by inducing the mitophagic process. Aging Cell. 2022 Jul

2. Franci L et al. MAPK15 controls cellular responses to oxidative stress by regulating NRF2 activity and expression of its downstream target genes. Redox Biol. 2024 Jun

Subcellular Location

Cytosol, Nucleus.

UNIPROT

SwissProt: Q8TD08 Human

SwissProt: Q80Y86 Mouse

SwissProt: Q9Z2A6 Rat

Synonyms

ERK 7 antibody

ERK 8 antibody

ERK-7 antibody

ERK-8 antibody

ERK7 antibody

ERK8 antibody

Extracellular regulated kinase 8 delta antibody

Extracellular signal regulated kinase 8 antibody

Extracellular signal-regulated kinase 7 antibody

Extracellular signal-regulated kinase 8 antibody

Expand

Images

  • Western blot analysis of ERK8 on different lysates with Rabbit anti-ERK8 antibody (<a href="/products/HA751144" style="font-weight: bold;text-decoration: underline;">HA751144</a>) at 1/2,000 dilution.<br /><br />Lane 1: Raji cell lysate (20 µg/Lane)<br />Lane 2: Mouse kidney tissue lysate (30 µg/Lane)<br />Lane 3: Mouse lung tissue lysate (30 µg/Lane)<br />Lane 4: Rat kidney tissue lysate (30 µg/Lane)<br />Lane 5: Rat lung tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 60 kDa<br />Observed band size: 55 kDa<br />Exposure time: Lane 1: 3 minutes; Lane 2-5: 4 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751144" style="font-weight: bold;text-decoration: underline;">HA751144</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ERK8 on different lysates with Rabbit anti-ERK8 antibody (HA751144) at 1/2,000 dilution.

    Lane 1: Raji cell lysate (20 µg/Lane)
    Lane 2: Mouse kidney tissue lysate (30 µg/Lane)
    Lane 3: Mouse lung tissue lysate (30 µg/Lane)
    Lane 4: Rat kidney tissue lysate (30 µg/Lane)
    Lane 5: Rat lung tissue lysate (30 µg/Lane)

    Predicted band size: 60 kDa
    Observed band size: 55 kDa
    Exposure time: Lane 1: 3 minutes; Lane 2-5: 4 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751144) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ERK8 antibody (<a href="/products/HA751144" style="font-weight: bold;text-decoration: underline;">HA751144</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751144" style="font-weight: bold;text-decoration: underline;">HA751144</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ERK8 antibody (HA751144) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751144) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ERK8 antibody (<a href="/products/HA751144" style="font-weight: bold;text-decoration: underline;">HA751144</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751144" style="font-weight: bold;text-decoration: underline;">HA751144</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ERK8 antibody (HA751144) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751144) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ERK8 antibody (<a href="/products/HA751144" style="font-weight: bold;text-decoration: underline;">HA751144</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751144" style="font-weight: bold;text-decoration: underline;">HA751144</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ERK8 antibody (HA751144) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751144) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"