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CYP11A1 Recombinant Rabbit Monoclonal Antibody [PSH07-26] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751147

CYP11A1 Recombinant Rabbit Monoclonal Antibody [PSH07-26] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CYP11A1 Recombinant Rabbit Monoclonal Antibody [PSH07-26] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CYP11A1 aa 1-350.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 60 kDa

Positive Control

HepG2 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse ovary tissue lysate, Mouse brain tissue lysate, Rat testis tissue lysate, Rat ovary tissue lysate.

Conjugation

unconjugated

Clone Number

PSH07-26

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Cholesterol side-chain cleavage enzyme is commonly referred to as P450scc, where "scc" is an acronym for side-chain cleavage. P450scc is a mitochondrial enzyme that catalyzes conversion of cholesterol to pregnenolone. This is the first reaction in the process of steroidogenesis in all mammalian tissues that specialize in the production of various steroid hormones. P450scc is a member of the cytochrome P450 superfamily of enzymes (family 11, subfamily A, polypeptide 1) and is encoded by the CYP11A1 gene.

Background References

1. Liu Y et al. Artemisinins ameliorate polycystic ovarian syndrome by mediating LONP1-CYP11A1 interaction. Science. 2024 Jun

2. Lin YC et al. Neurosteroidogenic enzymes: CYP11A1 in the central nervous system. Front Neuroendocrinol. 2021 Jul

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: P05108 Human

SwissProt: Q9QZ82 Mouse

SwissProt: P14137 Rat

Synonyms

Cholesterol 20 22 desmolase antibody

Cholesterol desmolase antibody

Cholesterol monooxygenase (side chain cleaving) antibody

Cholesterol side chain cleavage enzyme antibody

Cholesterol side chain cleavage enzyme mitochondrial antibody

Cholesterol side-chain cleavage enzyme antibody

CP11A_HUMAN antibody

CYP11A antibody

CYP11A1 antibody

CYPXIA1 antibody

Expand

Images

  • Western blot analysis of CYP11A1 on different lysates with Rabbit anti-CYP11A1 antibody (<a href="/products/HA751147" style="font-weight: bold;text-decoration: underline;">HA751147</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: Caco-2 cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: PC-12 cell lysate (20 µg/Lane)<br />Lane 5: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 6: Mouse ovary tissue lysate (40 µg/Lane)<br />Lane 7: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 8: Rat testis tissue lysate (40 µg/Lane)<br />Lane 9: Rat ovary tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 60 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751147" style="font-weight: bold;text-decoration: underline;">HA751147</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CYP11A1 on different lysates with Rabbit anti-CYP11A1 antibody (HA751147) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: Caco-2 cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: PC-12 cell lysate (20 µg/Lane)
    Lane 5: Mouse testis tissue lysate (40 µg/Lane)
    Lane 6: Mouse ovary tissue lysate (40 µg/Lane)
    Lane 7: Mouse brain tissue lysate (40 µg/Lane)
    Lane 8: Rat testis tissue lysate (40 µg/Lane)
    Lane 9: Rat ovary tissue lysate (40 µg/Lane)

    Predicted band size: 60 kDa
    Observed band size: 45 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"