CYP11A1 Recombinant Rabbit Monoclonal Antibody [PSH07-26]
Overview
Product Name
CYP11A1 Recombinant Rabbit Monoclonal Antibody [PSH07-26]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CYP11A1 aa 1-350.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 60 kDa
Positive Control
HepG2 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse ovary tissue lysate, Mouse brain tissue lysate, Rat testis tissue lysate, Rat ovary tissue lysate.
Conjugation
unconjugated
Clone Number
PSH07-26
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
| Chicken |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
Target
Function
Cholesterol side-chain cleavage enzyme is commonly referred to as P450scc, where "scc" is an acronym for side-chain cleavage. P450scc is a mitochondrial enzyme that catalyzes conversion of cholesterol to pregnenolone. This is the first reaction in the process of steroidogenesis in all mammalian tissues that specialize in the production of various steroid hormones. P450scc is a member of the cytochrome P450 superfamily of enzymes (family 11, subfamily A, polypeptide 1) and is encoded by the CYP11A1 gene.
Background References
1. Liu Y et al. Artemisinins ameliorate polycystic ovarian syndrome by mediating LONP1-CYP11A1 interaction. Science. 2024 Jun
2. Lin YC et al. Neurosteroidogenic enzymes: CYP11A1 in the central nervous system. Front Neuroendocrinol. 2021 Jul
Subcellular Location
Mitochondrion inner membrane.
Synonyms
Cholesterol 20 22 desmolase antibody
Cholesterol desmolase antibody
Cholesterol monooxygenase (side chain cleaving) antibody
Cholesterol side chain cleavage enzyme antibody
Cholesterol side chain cleavage enzyme mitochondrial antibody
Cholesterol side-chain cleavage enzyme antibody
CP11A_HUMAN antibody
CYP11A antibody
CYP11A1 antibody
CYPXIA1 antibody
ExpandImages
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Western blot analysis of CYP11A1 on different lysates with Rabbit anti-CYP11A1 antibody (HA722814) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: Caco-2 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Lane 5: Mouse testis tissue lysate (40 µg/Lane)
Lane 6: Mouse ovary tissue lysate (40 µg/Lane)
Lane 7: Mouse brain tissue lysate (40 µg/Lane)
Lane 8: Rat testis tissue lysate (40 µg/Lane)
Lane 9: Rat ovary tissue lysate (40 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 45 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722814) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Cholic acid inhibits ovarian steroid hormone synthesis and follicular development through farnesoid X receptor signaling in mice
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI: 10.1016/j.ijbiomac.2025.140458
IF: 8.5
Application: WB
Reactivity: Mouse
Publish date: 2025 Jan
-
Age-related changes in the mitochondrial, synthesis of steroids, and cellular homeostasis of the chicken ovary
Journal: Animal Reproduction Science
DOI: 10.1016/j.anireprosci.2024.107540
IF: 2.2
Application: WB
Reactivity: Chicken
Publish date: 2024 Jun