EPO Recombinant Rabbit Monoclonal Antibody [PSH07-33] - BSA and Azide free
Catalog# HA751154
EPO Recombinant Rabbit Monoclonal Antibody [PSH07-33] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
EPO Recombinant Rabbit Monoclonal Antibody [PSH07-33] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human EPO aa 1-193.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 21 kDa
Positive Control
293T transfected with EPO cell lysate.
Conjugation
unconjugated
Clone Number
PSH07-33
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Erythropoietin (EPO), also known as erythropoetin, haematopoietin, or haemopoietin, is a glycoprotein cytokine secreted mainly by the kidneys in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. Low levels of EPO (around 10 mU/mL) are constantly secreted in sufficient quantities to compensate for normal red blood cell turnover. Common causes of cellular hypoxia resulting in elevated levels of EPO (up to 10 000 mU/mL) include any anemia, and hypoxemia due to chronic lung disease and mouth disease.
Background References
1. Wu YW et al. Trial of Erythropoietin for Hypoxic-Ischemic Encephalopathy in Newborns. N Engl J Med. 2022 Jul
2. Sergio CM et al. Erythropoietin regulates signaling pathways associated with neuroprotective events. Exp Brain Res. 2022 May
Subcellular Location
Secreted.
UNIPROT
Synonyms
EP antibody
EPO alpha antibody
EPO antibody
Epoetin antibody
Erythropoetin antibody
Erythropoietin precursor antibody
MVCD2 antibody
Images
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☑ Cell treatment (CT)
This data was developed using HA722821, the same antibody clone in a different buffer formulation.
Western blot analysis of EPO on different lysates with Rabbit anti-EPO antibody (HA722821) at 1/1,000 dilution.
Lane 1: 293T transfected with empty control cell lysate
Lane 2: 293T transfected with EPO cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 25 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722821) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"