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EPO Recombinant Rabbit Monoclonal Antibody [PSH07-33]

Usd: 385

Specification

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Catalog# HA722821

EPO Recombinant Rabbit Monoclonal Antibody [PSH07-33]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

EPO Recombinant Rabbit Monoclonal Antibody [PSH07-33]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human EPO aa 1-193.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 21 kDa

Positive Control

293T transfected with EPO cell lysate.

Conjugation

unconjugated

Clone Number

PSH07-33

Reactivity Data

WB
Human
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Erythropoietin (EPO), also known as erythropoetin, haematopoietin, or haemopoietin, is a glycoprotein cytokine secreted mainly by the kidneys in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. Low levels of EPO (around 10 mU/mL) are constantly secreted in sufficient quantities to compensate for normal red blood cell turnover. Common causes of cellular hypoxia resulting in elevated levels of EPO (up to 10 000 mU/mL) include any anemia, and hypoxemia due to chronic lung disease and mouth disease.

Background References

1. Wu YW et al. Trial of Erythropoietin for Hypoxic-Ischemic Encephalopathy in Newborns. N Engl J Med. 2022 Jul

2. Sergio CM et al. Erythropoietin regulates signaling pathways associated with neuroprotective events. Exp Brain Res. 2022 May

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01588 Human

Synonyms

EP antibody

EPO alpha antibody

EPO antibody

Epoetin antibody

Erythropoetin antibody

Erythropoietin precursor antibody

MVCD2 antibody

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of EPO on different lysates with Rabbit anti-EPO antibody (<a href="/products/HA722821" style="font-weight: bold;text-decoration: underline;">HA722821</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T transfected with empty control cell lysate<br />Lane 2: 293T transfected with EPO cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722821" style="font-weight: bold;text-decoration: underline;">HA722821</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of EPO on different lysates with Rabbit anti-EPO antibody (HA722821) at 1/1,000 dilution.

    Lane 1: 293T transfected with empty control cell lysate
    Lane 2: 293T transfected with EPO cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 25 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722821) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"