Phospho-Cytosolic Phospholipase A2 (S505) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH07-56] (HA751176)
Catalog# HA751176
Rabbit Monoclonal to Phospho-Cytosolic Phospholipase A2 (S505)
Tested for
-
WB
-
Dot Blot
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA751176) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751176) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA751176) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751176) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.