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Phospho-Cytosolic Phospholipase A2 (S505) Recombinant Rabbit Monoclonal Antibody [PSH07-56]

Usd: 385

Specification

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Catalog# HA722849

Phospho-Cytosolic Phospholipase A2 (S505) Recombinant Rabbit Monoclonal Antibody [PSH07-56]

Application

  • WB

  • Dot Blot

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Phospho-Cytosolic Phospholipase A2 (S505) Recombinant Rabbit Monoclonal Antibody [PSH07-56]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser505 of human cPLA2 aa 491-540.

Species Reactivity

Human, Mouse

Validated Applications

WB, Dot Blot

Molecular Weight

Predicted band size: 85 kDa

Positive Control

HeLa cell lysate, RAW264.7 cell lysates.

Conjugation

unconjugated

Clone Number

PSH07-56

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • Dot Blot

  • 1:1,000

Target

Function

Cytosolic phospholipase A2 is an enzyme that in humans is encoded by the PLA2G4A gene. This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca2+ levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.

Background References

1. André S et al. Modulation of Cytosolic Phospholipase A2 as a Potential Therapeutic Strategy for Alzheimer\'s Disease. J Alzheimers Dis Rep. 2023 Dec

2. Zhao X et al. Cytosolic Phospholipase A2 Is Required for Fexofenadine\'s Therapeutic Effects against Inflammatory Bowel Disease in Mice. Int J Mol Sci. 2021 Oct

Subcellular Location

Cytoplasm, Golgi apparatus membrane, Nucleus envelope.

UNIPROT

SwissProt: P47712 Human

SwissProt: P47713 Mouse

Synonyms

Calcium dependent phospholipid binding protein antibody

CPLA 2 antibody

cPLA2 alpha antibody

cPLA2 antibody

Cytosolic phospholipase A2 antibody

Cytosolic phospholipase A2 group IVA antibody

Lysophospholipase antibody

MGC126350 antibody

PA24A_HUMAN antibody

Phosphatidylcholine 2 acylhydrolase antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (<a href="/products/HA722849" style="font-weight: bold;text-decoration: underline;">HA722849</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 85 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722849" style="font-weight: bold;text-decoration: underline;">HA722849</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722849) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 85 kDa
    Observed band size: 100 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722849) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on RAW264.7 cell lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (<a href="/products/HA722849" style="font-weight: bold;text-decoration: underline;">HA722849</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 85 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722849" style="font-weight: bold;text-decoration: underline;">HA722849</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on RAW264.7 cell lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722849) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 85 kDa
    Observed band size: 100 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722849) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Dot blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different proteins with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (<a href="/products/HA722849" style="font-weight: bold;text-decoration: underline;">HA722849</a>) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution for 1 hour at room temperature.<br /><br />Lane 1: Unmodified Cytosolic Phospholipase A2 (negative)<br />Lane 2: Phospho-Cytosolic Phospholipase A2 (S505) (positive)<br /><br />Proteins loading: 50ng, 25ng, 5ng, 1ng;<br /><br />Blocking and dilution buffer: 5% NFDM/TBST;<br /><br />Exposure time: 6 seconds; ECL: K1801.

    Dot blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different proteins with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722849) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature.

    Lane 1: Unmodified Cytosolic Phospholipase A2 (negative)
    Lane 2: Phospho-Cytosolic Phospholipase A2 (S505) (positive)

    Proteins loading: 50ng, 25ng, 5ng, 1ng;

    Blocking and dilution buffer: 5% NFDM/TBST;

    Exposure time: 6 seconds; ECL: K1801.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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