ASAH1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH07-71] (HA751183)
Catalog# HA751183
Rabbit Monoclonal to ASAH1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of ASAH1 on different lysates with Rabbit anti-ASAH1 antibody (HA751183) at 1/2,000 dilution.
Lane 1: SK-MEL-28 cell lysate
Lane 2: MDA-MB-231 cell lysate
Lane 3: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 37/55 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751183) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-MEL-28 cell lysate
Lane 2: MDA-MB-231 cell lysate
Lane 3: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 37/55 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751183) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.