ASAH1 Recombinant Rabbit Monoclonal Antibody [PSH07-71] - BSA and Azide free
Catalog# HA751183
ASAH1 Recombinant Rabbit Monoclonal Antibody [PSH07-71] - BSA and Azide free
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
ASAH1 Recombinant Rabbit Monoclonal Antibody [PSH07-71] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ASAH1 aa 1-395.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
SK-MEL-28 cell lysate, MDA-MB-231 cell lysate, SH-SY5Y cell lysate, human brain tissue, human kidney tissue.
Conjugation
unconjugated
Clone Number
PSH07-71
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
The ASAH1 gene encodes in humans the acid ceramidase enzyme. This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease and, recently, with a rare neurodegenerative condition known as spinal muscular atrophy with progressive myoclonic epilepsy. Two transcript variants encoding distinct isoforms have been identified for this gene. In melanocytic cells ASAH1 gene expression may be regulated by MITF.
Background References
1. Wander A et al. ASAH1 Variants Causing Spinal Muscular Atrophy Phenotype. Indian J Pediatr. 2023 Dec
2. Su Y et al. The interaction of ASAH1 and NGF gene involving in neurotrophin signaling pathway contributes to schizophrenia susceptibility and psychopathology. Prog Neuropsychopharmacol Biol Psychiatry. 2021 Jan
Subcellular Location
Lysosome, Secreted; Nucleus, Cytoplasm.
UNIPROT
Synonyms
AC antibody
ACDase antibody
Acid CDase antibody
Acid ceramidase antibody
Acid ceramidase precursor antibody
Acid ceramidase subunit beta antibody
Acylsphingosine deacylase antibody
ASAH 1 antibody
ASAH antibody
ASAH1 antibody
ExpandImages
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This data was developed using HA722887, the same antibody clone in a different buffer formulation.
Western blot analysis of ASAH1 on different lysates with Rabbit anti-ASAH1 antibody (HA722887) at 1/2,000 dilution.
Lane 1: SK-MEL-28 cell lysate
Lane 2: MDA-MB-231 cell lysate
Lane 3: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 37/55 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722887) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA722887, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ASAH1 antibody (HA722887) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722887) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722887, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ASAH1 antibody (HA722887) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722887) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"