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ASAH1 Recombinant Rabbit Monoclonal Antibody [PSH07-71]

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Specification

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Catalog# HA722887

ASAH1 Recombinant Rabbit Monoclonal Antibody [PSH07-71]

Application

  • WB

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

ASAH1 Recombinant Rabbit Monoclonal Antibody [PSH07-71]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ASAH1 aa 1-395.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 45 kDa

Positive Control

SK-MEL-28 cell lysate, MDA-MB-231 cell lysate, SH-SY5Y cell lysate, human brain tissue, human kidney tissue.

Conjugation

unconjugated

Clone Number

PSH07-71

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Not Recommended
Not Recommended
Rat
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000

Target

Function

The ASAH1 gene encodes in humans the acid ceramidase enzyme. This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease and, recently, with a rare neurodegenerative condition known as spinal muscular atrophy with progressive myoclonic epilepsy. Two transcript variants encoding distinct isoforms have been identified for this gene. In melanocytic cells ASAH1 gene expression may be regulated by MITF.

Background References

1. Wander A et al. ASAH1 Variants Causing Spinal Muscular Atrophy Phenotype. Indian J Pediatr. 2023 Dec

2. Su Y et al. The interaction of ASAH1 and NGF gene involving in neurotrophin signaling pathway contributes to schizophrenia susceptibility and psychopathology. Prog Neuropsychopharmacol Biol Psychiatry. 2021 Jan

Subcellular Location

Lysosome, Secreted; Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q13510 Human

Synonyms

AC antibody

ACDase antibody

Acid CDase antibody

Acid ceramidase antibody

Acid ceramidase precursor antibody

Acid ceramidase subunit beta antibody

Acylsphingosine deacylase antibody

ASAH 1 antibody

ASAH antibody

ASAH1 antibody

Expand

Images

  • Western blot analysis of ASAH1 on different lysates with Rabbit anti-ASAH1 antibody (<a href="/products/HA722887" style="font-weight: bold;text-decoration: underline;">HA722887</a>) at 1/2,000 dilution.<br /><br />Lane 1: SK-MEL-28 cell lysate<br />Lane 2: MDA-MB-231 cell lysate<br />Lane 3: SH-SY5Y cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 37/55 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722887" style="font-weight: bold;text-decoration: underline;">HA722887</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ASAH1 on different lysates with Rabbit anti-ASAH1 antibody (HA722887) at 1/2,000 dilution.

    Lane 1: SK-MEL-28 cell lysate
    Lane 2: MDA-MB-231 cell lysate
    Lane 3: SH-SY5Y cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 37/55 kDa

    Exposure time: 20 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722887) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ASAH1 antibody (<a href="/products/HA722887" style="font-weight: bold;text-decoration: underline;">HA722887</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722887" style="font-weight: bold;text-decoration: underline;">HA722887</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ASAH1 antibody (HA722887) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722887) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ASAH1 antibody (<a href="/products/HA722887" style="font-weight: bold;text-decoration: underline;">HA722887</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722887" style="font-weight: bold;text-decoration: underline;">HA722887</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ASAH1 antibody (HA722887) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722887) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"