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DNAJC6 Recombinant Rabbit Monoclonal Antibody [PSH07-95] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751197

DNAJC6 Recombinant Rabbit Monoclonal Antibody [PSH07-95] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

DNAJC6 Recombinant Rabbit Monoclonal Antibody [PSH07-95] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human DNAJC6 aa 714-913.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IHC-Fr, IF-Tissue

Molecular Weight

Predicted band size: 100 kDa

Positive Control

Human brain tissue lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

PSH07-95

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:200-1:1,000

  • IHC-Fr

  • 1:200-1:500

  • IF-Tissue

  • 1:200-1:500

Target

Function

Putative tyrosine-protein phosphatase auxilin is an enzyme that in humans is encoded by the DNAJC6 gene. DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc-finger domain.

Background References

1. Wulansari N et al. Neurodevelopmental defects and neurodegenerative phenotypes in human brain organoids carrying Parkinson\'s disease-linked DNAJC6 mutations. Sci Adv. 2021 Feb

2. Barsottini O et al. Commentary: Juvenile Dystonia-Parkinsonism due to DNAJC6 Mutation. Mov Disord Clin Pract. 2021 Sep

Subcellular Location

Cytoplasmic vesicle, clathrin-coated vesicle.

UNIPROT

SwissProt: O75061 Human

SwissProt: Q80TZ3 Mouse

Entrez Gene: 313409 Rat

Synonyms

auxilin antibody

DJC6 antibody

DnaJ (Hsp40) homolog, subfamily B, member 6 antibody

DnaJ (Hsp40) homolog, subfamily C, member 6 antibody

DnaJ homolog subfamily C member 6 antibody

EC 3.1.3.48 antibody

KIAA0473 antibody

MGC129914 antibody

MGC129915 antibody

MGC48436 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of DNAJC6 on different lysates with Rabbit anti-DNAJC6 antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: Human brain tissue lysate<br />Lane 2: Human liver tissue lysate (negative)<br />Lane 3: Mouse brain tissue lysate<br />Lane 4: Mouse cerebellum tissue lysate<br />Lane 5: Mouse liver tissue lysate (negative)<br />Lane 6: Rat brain tissue lysate<br />Lane 7: Rat liver tissue lysate (negative)<br />Lane 8: Rat cerebellum tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 100 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: Lane 1-8 (left): 30 seconds; Lane 1-8 (right): 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of DNAJC6 on different lysates with Rabbit anti-DNAJC6 antibody (HA751197) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: Human brain tissue lysate
    Lane 2: Human liver tissue lysate (negative)
    Lane 3: Mouse brain tissue lysate
    Lane 4: Mouse cerebellum tissue lysate
    Lane 5: Mouse liver tissue lysate (negative)
    Lane 6: Rat brain tissue lysate
    Lane 7: Rat liver tissue lysate (negative)
    Lane 8: Rat cerebellum tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 100 kDa
    Observed band size: 110 kDa

    Exposure time: Lane 1-8 (left): 30 seconds; Lane 1-8 (right): 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751197) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-DNAJC6 antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-DNAJC6 antibody (HA751197) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751197) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-DNAJC6 antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-DNAJC6 antibody (HA751197) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751197) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-DNAJC6 antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-DNAJC6 antibody (HA751197) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751197) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-DNAJC6 antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-DNAJC6 antibody (HA751197) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751197) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-DNAJC6 antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-DNAJC6 antibody (HA751197) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751197) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Rabbit anti-DNAJC6 antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751197" style="font-weight: bold;text-decoration: underline;">HA751197</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Rabbit anti-DNAJC6 antibody (HA751197) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751197) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Liver (negative)<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Not required

    ☑ Relative expression (RE)

    Application: IHC-Fr

    Species: Mouse

    Site: Liver (negative)

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Not required

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"