Skip to content

EXOSC9 Recombinant Rabbit Monoclonal Antibody [PSH08-55] - BSA and Azide free

Usd: 649

Specification

Bulk Order Quote | Customization Request

Catalog# HA751230

EXOSC9 Recombinant Rabbit Monoclonal Antibody [PSH08-55] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

EXOSC9 Recombinant Rabbit Monoclonal Antibody [PSH08-55] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human EXOSC9 aa 1-350.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 49 kDa

Positive Control

293T cell lysate, HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate.

Conjugation

unconjugated

Clone Number

PSH08-55

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Exosome component 9, also known as EXOSC9, is a human gene, the protein product of which (sometimes called RRP45 or PM/Scl-75) is part of the exosome complex and is an autoantigen is patients with certain auto immune diseases, most notably scleromyositis.

Background References

1. Yoshino S et al. EXOSC9 depletion attenuates P-body formation, stress resistance, and tumorigenicity of cancer cells. Sci Rep. 2020 Jun

2. Quttina M et al. Exosc9 Initiates SUMO-Dependent lncRNA TERRA Degradation to Impact Telomeric Integrity in Endocrine Therapy Insensitive Hormone Receptor-Positive Breast Cancer. Cells. 2023 Oct

Subcellular Location

Cytoplasm, Nucleus, nucleolus, nucleoplasm.

UNIPROT

SwissProt: Q06265 Human

SwissProt: Q9JHI7 Mouse

SwissProt: Q4QR75 Rat

Synonyms

Autoantigen PM/Scl 1 antibody

EXOS9_HUMAN antibody

EXOSC 9 antibody

EXOSC9 antibody

Exosome complex component RRP45 antibody

Exosome complex exonuclease RRP45 antibody

Exosome component 9 antibody

p5 antibody

p6 antibody

P75 polymyositis scleroderma overlap syndrome associated autoantigen antibody

Expand

Images

  • This data was developed using <a href="/products/HA723001" style="font-weight: bold;text-decoration: underline;">HA723001</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of EXOSC9 on different lysates with Rabbit anti-EXOSC9 antibody (<a href="/products/HA723001" style="font-weight: bold;text-decoration: underline;">HA723001</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: NIH/3T3 cell lysate<br />Lane 6: C2C12 cell lysate<br />Lane 7: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 49 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723001" style="font-weight: bold;text-decoration: underline;">HA723001</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA723001, the same antibody clone in a different buffer formulation.
    Western blot analysis of EXOSC9 on different lysates with Rabbit anti-EXOSC9 antibody (HA723001) at 1/2,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: NIH/3T3 cell lysate
    Lane 6: C2C12 cell lysate
    Lane 7: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 49 kDa
    Observed band size: 70 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723001) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"